Acta Botanica Croatica, Vol. 49 No. 1, 1990.
Original scientific paper
Standardizing the Conditions for Performance of Immunoelectrophoretic Experiments with Tobacco Streak Ilarvirus Particles
Mladen Krajačić
; Hrvatska
Zlata Štefanac
; Hrvatska
Abstract
The 0.016 M natrium phosphate pH 7.0 as a gel-buffer, of the two-fold molarity in the LKB-Gelman 6800 A—1 apparatus vessels, ensured the optimal immuno-electro- phoretic patterns of tobacco streak ilarvirus (TSV/Cle) particles when a constant current of 4 mA and a voltage of 20 V/cm gel were applied for 4 h at 2°C. The conditions prooved adequate in demonstrating the electrophoretic heterogeneity of unfixed TSV particles.
Keywords
Hrčak ID:
159550
URI
Publication date:
31.12.1990.
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