Original scientific paper

In vitro Multiple Shoot Induction and Plant Regeneration in Betida pendula

Višnja Besendorfer ; Hrvatska
Branka Kolevska-Pletikapić ; Hrvatska

Abstract

Shoot development and plant regeneration through axillary bud culture of the European birch (Betula pendula Roth.) were successfully achieved. The modified ACM medium supplemented with 2.2 μM BA was used as initial medium. Both adventitious and axillary shoot development was induced in 2-year-old genotype explants, while in 4- year-old genotype cultures only axillary shoots developed. The establishment of multiple shoot culture was genotype depending. It was found that ACM nutrition medium, supplemented with 2.2 μM BA, was more effective for shoot multiplication than WPM and BTM. Microcuttings were easily rooted on ACM medium supplemented with 1.0 μM IBA. The complete plants obtained were successfully transferred to soil.

Keywords

Hrčak ID:

159559

URI

https://hrcak.srce.hr/159559

Publication date:

31.12.1990.

Article data in other languages: croatian

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