Acta Botanica Croatica, Vol. 50 No. 1, 1991.
Original scientific paper
Glycerol Efficiently lessens Tobacco Streak Ilarvirus Particles Aggregation during Formaldehyde Fixation
Zlata Štefanac
; Hrvatska
Mladen Krajačić
; Hrvatska
Abstract
We describe the use of glycerol to reduce aggregation of tobacco streak ilarvirus (TSV) particles caused by formaldehyde (FA) fixation. After treatment of partially purified TSV preparations with 1% FA in the presence of 10% glycerol, double radial immunodiffusion tests revealed the presence of two to four times more reacting virus than in preparations treated with 1% FA only. Consequently, the method provides higher quality preparations intended for different immunochemical purposes and biophysical and biochemical analyses. We expect it to be useful in fixation of viruses which tend to aggregate in the presence of FA.
Keywords
Hrčak ID:
159701
URI
Publication date:
31.12.1991.
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