Acta Botanica Croatica, Vol. 50 No. 1, 1991.
Original scientific paper
Nuclear Changes in European Black Pine Seedlings caused by Growth Regulators
Dražena Papeš
; Hrvatska
Višnja Besendorfer
; Hrvatska
Mirjana Pavlica
; Hrvatska
Abstract
Cytogenetic effects caused by two synthetic auxins: 1-naphtaleneacetic acid (NAA) and indole-3-butyric acid (IBA) as well as by synthetic cytokinin benzyladenine (BA), on root tip cells of ten-day-old European black pine (Pinus nigra Arn.) seedlings were observed. The results showed that auxins induced stronger cytogenetic effects than cytokinin. After treatment with auxins the mitotic activity and the corresponding number of chromosomal aberrations increased in parallel to the concentrations used. Both auxins, NAA and IBA, induced almost the same types of nuclear changes such as spindle failure, chromosome fragmentation, laggards and polyploidy. NAA induced a higher frequency of chromosomal changes than IBA. Also, the recovery period after the application of NAA was much slower as compared to that of IBA. Cytokinin BA showed the opposite effect. After treatment with the highest concentration the mitotic activity was very similar to the control, but after the recovery period the mitotic index increased significantly. This was also the case with a number of aberrant cells.
Keywords
Hrčak ID:
159705
URI
Publication date:
31.12.1991.
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