Original scientific paper

Contribution to the study of the changes in the gills of zebrafish (Danio rerio) when exposed to dioxin

Jose M. NAVAS orcid.org/0000-0002-9425-5893 ; Department of Comparative Anatomy and Pathology, University of Córdoba. Rabanales Campus. Faculty of Veterinary Carretera Madrid- Cádiz s/n, 14071 Córdoba, Spain
Andres M. DIZ orcid.org/0000-0001-7079-3868 ; Department of Comparative Anatomy and Pathology, University of Córdoba. Rabanales Campus. Faculty of Veterinary Carretera Madrid- Cádiz s/n, 14071 Córdoba, Spain
Rosario MOYANO ; Department of Pharmacology, Toxicology and Forensic Medicine. University of Córdoba. Rabanales Campus. Faculty of Veterinary Carretera Madrid-Cádiz s/n, 14071 Córdoba, Spain
Jose M. VÁZQUEZ-AUTÓN ; Department of Comparative Anatomy and Pathology. University of Murcia. Espinardo Campus. Faculty of Veterinary Medicine. 30100 Murcia, Spain
Maria D. AYALA ; Department of Comparative Anatomy and Pathology. University of Murcia. Espinardo Campus. Faculty of Veterinary Medicine. 30100 Murcia, Spain
Jose GARCÍA-MONTERDE ; Department of Comparative Anatomy and Pathology, University of Córdoba. Rabanales Campus. Faculty of Veterinary Carretera Madrid- Cádiz s/n, 14071 Córdoba, Spain
Alfonso BLANCO ; Department of Comparative Anatomy and Pathology, University of Córdoba. Rabanales Campus. Faculty of Veterinary Carretera Madrid- Cádiz s/n, 14071 Córdoba, Spain

Abstract

This study determined cell changes in the gills of zebrafish (Danio rerio, Hamilton, 1822) as a result of a dietary supply of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). 50 zebrafish were divided into five groups, one control group and four experimental ones. The experimental groups were supplied 10, 40, 100 and 270 ng/day/fish TCDD, respectively. Structural, ultrastructural and morphometric studies of the fish gill were carried out, indicating that, in low concentrations, fish gills presented hyperemia with progressive erosion of the endothelium of the capillaries resulting in edema and microhaemorrhages. In the non-respiratory gill, in those groups with low concentrations, it could be observed that the chloride cells presented hypertrophy as a result of dilatation of the intracellular canal and swelling of the microvilli, losing their activity in the degradation of chemical substances (according to some authors) in those groups with a higher concentration. Mucous cells presented hyperplasia and hypertrophy. The sensory buds were not directly affected, but, due to the presence of interstitial edema, swelling of the supporting cells occurred without the involvement of the neurosensory cells. We concluded that dioxins affect the gills to a greater or lesser extent depending on their concentration in the fish’s diet, which alters their functionality.

Keywords

PCB; gill; histopathology; ultrastructure; cell morphology

Hrčak ID:

171652

URI

https://hrcak.srce.hr/171652

Publication date:

12.12.2016.

Article data in other languages: croatian

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