Original scientific paper
https://doi.org/10.15255/KUI.2016.053
Fast Separation and Determination of Flavonoids in Honey Samples by Capillary Zone Electrophoresis
Jian-Qiu Tu
; Henan Institute of Science and Technology, Xinxiang 453 003, Kina
Zhong-Yin Zhang
; Henan Institute of Science and Technology, Xinxiang 453 003, Kina
Cheng-Xing Cui
; Henan Institute of Science and Technology, Xinxiang 453 003, Kina
Meng Yang
; Henan Institute of Science and Technology, Xinxiang 453 003, Kina
Ying Li
; Henan Institute of Science and Technology, Xinxiang 453 003, Kina
Yu-Ping Zhang
; Henan Institute of Science and Technology, Xinxiang 453 003, Kina
Abstract
Flavonoids have crucial applications in the biological and physiological fields. Honey, as an important sweet food made by bees, is rich in flavonoids. In this paper, the analytical method for flavonoids determination in different sorts of honey from different geographical locations was developed by capillary zone electrophoresis with direct ultraviolet detection. With a running buffer (borate, 20 mmol l–1) at pH of 8.4, four typical flavonoids, rutin, quercetin, kaempferol and myricetin, were separated in five minutes under a applied potential of 25 kV. A linear relationship within the range of 2.0 – 500 mg l–1 was found for these four kinds of flavonoids. Moreover, the detection limits ranged from 1.17 to 1.76 mg l–1. The recoveries lie in the range between 80 % – 107 %. The developed method was then used in the separation and determination of flavonoids in real honey samples collected from 12 geographical locations in the Henan Province of China. Rutin was detected in six, and quercetin in eight honey samples, which may be the markers for the identification of honey from different geographical origins.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords
capillary zone electrophoresis; flavonoids; honey; UV detection
Hrčak ID:
177615
URI
Publication date:
1.4.2017.
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