Preliminary communication
https://doi.org/10.15255/KUI.2017.017
Immobilization of Tyrosinase on (3-Aminopropyl)triethoxysilane-Functionalized Carbon Felt-Based Flow-Through Detectors for Electrochemical Detection of Phenolic Compounds
Zheng Zhou
; School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Road, Hi-tech zone, Anshan, Liaoning, 114 501, Kina
Yue Wang
; School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Road, Hi-tech zone, Anshan, Liaoning, 114 501, Kina
Zhiqiang Zhang
; School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Road, Hi-tech zone, Anshan, Liaoning, 114 501, Kina
Yan Zhang
; School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Road, Hi-tech zone, Anshan, Liaoning, 114 501, Kina
Yasushi Hasebe
; Department of Life Science and Green Chemistry, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama, 369-0293, Japan
Yuming Song
; School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Road, Hi-tech zone, Anshan, Liaoning, 114 501, Kina
Cuiping Wang
; School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Road, Hi-tech zone, Anshan, Liaoning, 114 501, Kina
Abstract
Tyrosinase (TYR) was covalently immobilized onto amino-functionalized carbon felt (CF) surface via glutaraldehyde (GA). Prior to the TYR-immobilization, primary amino group was introduced to the CF surface by treatment with 3-aminopropyltriethoxysilane (APTES). The resulting TYR-immobilized CF was used as a working electrode unit of an electrochemical flow-through detector for mono- and di-phenolic compounds (i.e., catechol, p-cresol, phenol and p-chlorophenol). Additionally, flow injection peaks based on electroreduction of the enzymatically produced o-quinone species were detected at −0.05 V vs. Ag/AgCl. The resulting TYR/GA/APTES/CF biosensor responded well to all compounds tested with limits of detection range from 7.5 to 35 nmol l–1 (based on three times S/N ratio). Moreover, such modified electrode exhibits good stability and reproducibility for catechol. No serious degradation of the peak current was found over 30 consecutive injections.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords
tyrosinase; carbon felt; (3-aminopropyl)triethoxysilane; flow-through detector
Hrčak ID:
185074
URI
Publication date:
31.7.2017.
Visits: 1.125 *