Conference paper

The Mode of Binding of Carbon Monoxide to Iron in Cytochrome P450 and Cytochrome P420. An Infrared Study

H. Rein ; Department of Methods and Theory and Department of Biocatalysis, Central Institute of Molecular Biology, Acader;iy of Sciences of the GDR, 1115 Berlin, GDR
S. Bohm ; Department of Methods and Theory and Department of Biocatalysis, Central Institute of Molecular Biology, Acader;iy of Sciences of the GDR, 1115 Berlin, GDR
G.-R. Janig ; Department of Methods and Theory and Department of Biocatalysis, Central Institute of Molecular Biology, Acader;iy of Sciences of the GDR, 1115 Berlin, GDR
K. Ruckpaul ; Department of Methods and Theory and Department of Biocatalysis, Central Institute of Molecular Biology, Acader;iy of Sciences of the GDR, 1115 Berlin, GDR

Abstract

The infrared stretching vibrations (vc0 ) of the CO-complexes
of cytochrome P450 and cytochrome P420 have been determined
from the infrared difference spectra. The CO-complexes exhibit
ir-bands at 1949 cm-1 and 1966 cm-1 with half widths of -17 cm·1
and - 20 cm·1 respectively. These results are con;ipared with the
CO-stretching frequencies of other hemoproteins and discussed with
respect to specific interactions of the CO-ligand with the protein
moiety and to the ligand trans to CO in the cytochrome.

Keywords

Hrčak ID:

196372

URI

https://hrcak.srce.hr/196372

Publication date:

30.3.1977.

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