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Chemistry in Industry : Journal of Chemists and Chemical Engineers of Croatia, Vol. 67 No. 7-8, 2018.

Original scientific paper

https://doi.org/10.15255/KUI.2017.048

Production of Bioethanol from Corn Cobs

Mirela Ivančić Šantek orcid id orcid.org/0000-0002-5935-6672 ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia
Ines Zvonar ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia
Sunčica Beluhan orcid id orcid.org/0000-0003-0138-6286 ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia
Božidar Šantek orcid id orcid.org/0000-0001-9583-534X ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia

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Abstract

Bioethanol was produced from cellulase-hydrolysed pretreated corn cobs by simultaneous saccharification and fermentation (SSF) with yeast Saccharomyces cerevisiae. The effect of the duration of the corn cob prehydrolysis step on ethanol yield by SSF process was studied and compared to the conventional SSF process. Two mixtures of commercially available enzyme preparations were used for biomass hydrolysis; the first contained Celluclast 1.5L (Sigma) and ß-glucosidase (Carl Roth), and the second mixture contained Celluclast 1.5L (Sigma) and Viscozyme L (Sigma). Two days of prehydrolysis had a positive effect on ethanol yield, while the yield decreased with longer prehydrolysis. The highest ethanol concentration of 41.24 g dm–3 was obtained by SSF process with two days of prehydrolysis, which corresponded to 63.15 % of the theoretical yield based on untreated corn cobs. However, the highest total productivity of 0.36 g dm–3 h–1 was observed in conventional SSF process using enzyme mixture containing Celluclast 1.5L (Sigma) and Viscozyme L (Sigma). Concentration of ethanol was 36.6 g dm–3 which corresponded to 56.02 % of the theoretical yield based on untreated corn cobs.


This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

corn cobs; bioethanol; cellulases; simultaneous saccharification and fermentation; prehydrolysis

Hrčak ID:

203658

URI

https://hrcak.srce.hr/203658

Publication date:

16.7.2018.

Article data in other languages: croatian

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