Original scientific paper
https://doi.org/10.15644/asc52/4/2
In Vitro Biocompatibility of Preheated Giomer and Microfilled-Hybrid Composite
Alena Knežević
; Division of Restorative Sciences, Herman Ostrow School of Dentistry, University of Southern California, Norris Dental Science Center, 925 W 34th St, Los Angeles, CA 90089, USA
Davor Želježić
; Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska c. 2, 10000 Zagreb, Croatia
Nevenka Kopjar
; Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska c. 2, 10000 Zagreb, Croatia
Sillas Duarte, Jr.
; Division of Restorative Sciences, Herman Ostrow School of Dentistry, University of Southern California, Norris Dental Science Center, 925 W 34th St, Los Angeles, CA 90089, USA
Zrinka Tarle
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Gunduliceva 5, 10000 Zagreb, Croatia
Abstract
Objective: The aim of this study was to evaluate cytotoxic potencies of two light cured composite materials after heating on different temperatures and cured directly and through CAD/CAM overlay. Materials and methods: Composite materials (microfilled-hybrid Gradia Direct Posterior and Beautifil II) were heated in a Calset warming unit at three different temperatures (T1:37°C, T2:54°C, T3:68°C). A small amount of heated composite material was placed in a round mold (diameter 6mm; 0.65mm thick), covered with Mylar sheet, pressed and polymerized with Bluephase LED unit. One group of samples were polymerized directly, and the other group through 2mm thick CAD/CAM ceramic-reinforced polymer (CRP) and CAD/CAM lithium disilicate ceramic (LDC) overlay for 20 and 40 seconds. The polymerized samples were placed immediately after curing in a lymphocyte cell culture. The viability of peripheral blood lymphocytes was evaluated using a dye exclusion technique by simultaneous staining with ethidium bromide and acridine orange. Quantitative assessments were made by determination of the percentage of viable, apoptotic and necrotic cells. The Pearson chi-square test was used for statistical analysis. Results: In case of 20 seconds polymerization, the highest number of viable cells polymerization were recorded when materials were heated at 37°C (T1), while in case of 40 seconds polymerization, the highest number of viable cells were recorded when the materials were heated at 54°C (T2). The samples polymerized through CAD/CAM overlays showed less cytotoxicity than samples polymerized directly. Conclusion: Apart from composite material composition, the cell viability was also influenced by curing time, temperature of pre-heating and polymerization pattern.
Keywords
Cytotoxicity; Pre-heating of Composites; Temperature; Composite Material
Hrčak ID:
213453
URI
Publication date:
19.12.2018.
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