Original scientific paper

https://doi.org/10.11613/BM.2019.010706

Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

Tomás Barranco ; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Campus of Excellence Mare Nostrum, University of Murcia, Murcia, Spain
Camila P Rubio ; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Campus of Excellence Mare Nostrum, University of Murcia, Murcia, Spain
Asta Tvarijonaviciute ; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Campus of Excellence Mare Nostrum, University of Murcia, Murcia, Spain
Mónica Rubio ; Department of Animal Medicine and Surgery, University CEU Cardenal Herrera, Valencia, Spain
Elena Damia ; Department of Animal Medicine and Surgery, University CEU Cardenal Herrera, Valencia, Spain
Elsa Lamy ; Institute of Mediterranean Agricultural and Environmental Sciences (ICAAM), University of Évora, Évora, Portugal
Ramón Cugat ; Arthroscopy and Traumatology Unit of the Quirón Hospital, Barcelona, Spain
José J Cerón ; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Campus of Excellence Mare Nostrum, University of Murcia, Murcia, Spain
Fernando Tecles ; Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Campus of Excellence Mare Nostrum, University of Murcia, Murcia, Spain
Damián Escribano ; Department of Animal and Food Science, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Barcelona, Spain

Abstract

Introduction: In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions.
Materials and methods: Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase
(AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing
ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen
peroxide (H2O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6,
24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at – 20 ºC and – 80 ºC.
Results: At RT, changes appeared at 6 hours for TEA and H2O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP.
At 4 ºC changes were observed after 6 hours for TEA and H2O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At – 20
ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2O2. At – 80 ºC observed changes were after 3 months for TEA
and H2O2.
Conclusions: In short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more
stable at - 80 ºC than at – 20 ºC.

Keywords

biomarker; enzyme stability; oxidative stress; saliva; temperature

Hrčak ID:

217218

URI

https://hrcak.srce.hr/217218

Publication date:

15.2.2019.

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