Original scientific paper
https://doi.org/10.15567/mljekarstvo.2019.0206
Management of blood lipid profile and oxidative status in Holstein and Simmental dairy cows during lactation
Dejan Obućinski
; University Business Academy, Faculty of Economics and Engineering Management, Department of Engineering Management in Biotechnology, Cvećarska 2, 21000 Novi Sad, Serbia
Dragan Soleša
orcid.org/0000-0003-1185-7969
; University Business Academy, Faculty of Economics and Engineering Management, Department of Engineering Management in Biotechnology, Cvećarska 2, 21000 Novi Sad, Serbia
Denis Kučević
orcid.org/0000-0002-1932-7156
; University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia
Radivoj Prodanović
orcid.org/0000-0002-7088-8506
; University Business Academy, Faculty of Economics and Engineering Management, Department of Engineering Management in Biotechnology, Cvećarska 2, 21000 Novi Sad, Serbia
Mirela Tomaš Simin
orcid.org/0000-0003-1833-9857
; University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia
Dragana Ljubojević Pelić
; Scientific Institute of Veterinary Medicine “Novi Sad”, Rumenački put 20, 21000 Novi Sad, Serbia
Olivera Đuragić
orcid.org/0000-0003-3189-5057
; Scientific Institute for Food Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
Nikola Puvača
orcid.org/0000-0002-5500-7010
; University Business Academy, Faculty of Economics and Engineering Management, Department of Engineering Management in Biotechnology, Cvećarska 2, 21000 Novi Sad, Serbia
Abstract
Managing and determination of the blood lipid profile and oxidative status are useful tool to show the influence of foreign chemical substances on production of dairy cows. Investigation of various biomarkers of oxidative stress related to various problems in the production dairy cows presents a very important issue. The aim of this research was to investigate different biological markers in two different breeds of dairy cows – the Holstein Friesian and the Simmental, during different stages of lactation. The total of 60 Holstein Friesian and Simmental cows were divided into two treatments (HF and S) which was further divided into three groups each, respectively. Lactating groups of each cow breed was divided into three stages at early lactation of 32-75 days (HF1 and S1), peak lactation of 80-165 days (HF2 and S2) and low lactation of 175 days onward (HF3 and S3). During the research the total milk production and the percentage of milk fat and protein were determined. Blood samples were collected from jugular vein of each cow, the serum was harvested without anticoagulant and stored at -20 °C in small aliquots till further analysis. Serum was then analyzed for triglyceride, cholesterol, HDL, LDL, AST, ALT, GGT, the total antioxidant status (TAS), the total oxidant status (TOS), MDA, SOD, catalase and paraoxonase (PON1). Significantly (P<0.05) higher values of cholesterol, TOS and SOD at peak lactation stage was observed in all cows. A gradual decline from early lactation to low lactation was observed in the values of triglycerides and ALT, while MDA gradually increased from the same stage in all cows. The values of LDL and catalase showed an irregular pattern of variations in various lactation stages among all groups. The Holstein Friesian breed showed significantly higher values of ALT and PON1, while values of HDL, TAS and TOS were the lowest in Simmental group (P<0.05). By measuring the signs of oxidative stress, it was concluded that metabolic efforts of cows during the peak of lactation affected the investigated parameters. Disturbance in feed intake and rise in metabolic processes lift up oxidative stress in cows, especially during the peak of lactation, which might be determined by monitoring blood alterations. Results of our study indicated that the Holstein Friesian breed was under higher oxidative stress during different lactation stages compared to Simmental dairy cows.
Keywords
management; cows; lactation; milk production; stress; biomarkers
Hrčak ID:
218655
URI
Publication date:
1.4.2019.
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