Original scientific paper
https://doi.org/10.17113/ftb.57.02.19.5823
Comparative Study of Inhibitory Potential of Dietary Phytochemicals Against Quorum Sensing Activity of and Biofilm Formation by Chromobacterium violaceum 12472, and Swimming and Swarming Behaviour of Pseudomonas aeruginosa PAO1
Elif Burcu Bali
; Gazi University, Vocational School of Health Services, Department of Medical Services and Techniques, Programme of Medical Laboratory Techniques, 06830 Gölbaşı, Ankara, Turkey
Kübra Erkan Türkmen
; Hacettepe University, Faculty of Science Department of Biology and Biotechnology, 06800 Beytepe, Ankara, Turkey
Demet Erdönmez
; Aksaray University, Faculty of Science and Letters, Department of Biology, 68100 Aksaray, Turkey
Necdet Sağlam
; Hacettepe University, Graduate School of Science and Engineering, Nanotechnology and Nanomedicine Division, 06800 Beytepe, Ankara, Turkey
Abstract
Quorum sensing (QS) and biofilm formation are important mechanisms related to antibiotic resistance of many pathogens. Alternative treatments are needed to prevent recurrent or chronic infections caused by multi-resistant pathogens. Therefore, the aim of this study is to investigate and compare the inhibitory potential of the dietary phytochemicals: curcumin, quercetin, apigenin, pyrogallol, gallic acid and luteolin against QS of and biofilm formation by Chromobacterium violaceum ATCC 12472 and the swimming and swarming abilities of Pseudomonas aeruginosa PAO1. Anti-QS potential of the phytochemicals was evaluated qualitatively and quantitatively using C. violaceum via the disk diffusion assay based on violacein pigment inhibition at the subminimal inhibitory concentrations ranging from 46.87 to 750 μg/mL. The results of anti-QS and antibiofilm activities on C. violaceum demonstrated that all the phytochemicals except pyrogallol and gallic acid inhibited violacein production (from (11.0±0.1) to (88.2±0.1) %) in a concentration-dependent manner. In addition, the biofilm formation was also significantly inhibited (p<0.05) in the presence of all the phytochemicals ((1.38±0.08)–(84.2±0.2) %). In the present study, the results revealed that quercetin, curcumin, apigenin and luteolin could be promising QS and biofilm inhibitory agents against the C. violaceum 12472 biosensor system. Our findings also suggest that all the phytochemicals, especially curcumin, quercetin and pyrogallol, might be anti-pathogenic agents against P. aeruginosa PAO1 infections due to the ability to control QS. However, more comprehensive studies at the molecular level, explaining their anti-QS mechanisms, need to be conducted to confirm these results and identify the genes involved.
Keywords
phytochemicals; anti-quorum sensing; Chromobacterium violaceum ATCC 12472; Pseudomonas aeruginosa PAO1; antibiofilm activity
Hrčak ID:
223875
URI
Publication date:
30.6.2019.
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