Original scientific paper
https://doi.org/10.2478/aiht-2019-70-3322
Toxic activity of Prunus spinosa L. flower extract in hepatocarcinoma cells
Teuta Murati
; Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Marina Miletić
; Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Josipa Kolarić
; Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Vanja Lovrić
; Laboratory for Technology of Fruits and Vegetables Preservation and Processing, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Danijela Bursać Kovačević
; Laboratory for Technology of Fruits and Vegetables Preservation and Processing, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Predrag Putnik
; Laboratory for Technology of Fruits and Vegetables Preservation and Processing, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Irena Landeka Jurčević
; Laboratory for Food Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Domagoj Đikić
; Division of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Verica Dragović-Uzelac
; Laboratory for Technology of Fruits and Vegetables Preservation and Processing, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Ivana Kmetič
; Laboratory for Toxicology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Abstract
Prunus spinosa L. (blackthorn) is used in traditional medicine as a remedy for various diseases. To establish its anticancer properties, we exposed human liver cancer cells (Hep G2) to a range of blackthorn flower extract concentrations (10-200 μg/mL) and determined cytotoxic activity with the neutral red and kenacid blue methods after 24, 48, and 72 h of incubation. Statistically significant inhibitory effects on Hep G2 cellular proliferation were observed at concentrations above 50 μg/mL (p<0.001–0.05). Cell viability was lower when determined with neutral red than kenacid blue method. In addition, we evaluated antioxidant/ prooxidant effects of the blackthorn flower extract by measuring reactive oxygen species (ROS), and the results confirmed its prooxidant behaviour within the applied concentration range. Flow cytometry determined primarily necrotic and apoptotic cell death, which provides additional evidence of its cytotoxic effect on liver carcinoma.
Keywords
blackthorn; cytotoxicity; Hep G2; kenacid blue; neutral red; ROS
Hrčak ID:
229324
URI
Publication date:
6.12.2019.
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