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Veterinary Archives, Vol. 90 No. 1, 2020.

Original scientific paper

https://doi.org/10.24099/vet.arhiv.0477

The prevalence of four virulence genes in strains of Campylobacter jejuni isolated from broilers in Serbia

Jelena Jovanović orcid id orcid.org/0000-0003-0301-729X ; Institute of Meat Hygiene and Technology, Belgrade, Serbia
Ivana Branković-Lazić ; Institute of Meat Hygiene and Technology, Belgrade, Serbia
Tatjana Baltić orcid id orcid.org/0000-0002-0496-3133 ; Institute of Meat Hygiene and Technology, Belgrade, Serbia
Milica Jovanović ; Department of Microbiology, Clinical Centre of Serbia, Belgrade, Serbia
Vesna Đorđević ; Institute of Meat Hygiene and Technology, Belgrade, Serbia
Vlado Teodorović ; Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia
Branko Velebit orcid id orcid.org/0000-0002-7577-8074 ; Institute of Meat Hygiene and Technology, Belgrade, Serbia

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Abstract

Campylobacter jejuni is a major cause of human diarrheal disease. The objective of this research study was to determine the prevalence of different virulence genes in isolates recovered from broiler meat at slaughterhouses in Serbia. Out of 115 Campylobacter spp. samples recovered, a total of 35 isolates were identified as C. jejuni on the basis of morphological, biochemical-based detection, multiplex PCR, and sequencing of the highly conserved region of the dnaJ gene encoding the DnaJ Hsp40 family protein. The isolates were screened for the presence of four pathogenic genes, namely flaA, cadF, cdtB, and cgtB, which are responsible for the expression of adherence, colonization, cytotoxin production in C. jejuni, and the onset of Guillain-Barre syndrome. The isolates showed a wide variation in the presence of these genes. All the isolates were positive for flaA. Furthermore, a high genetic heterogeneity in the C. jejuni population was found in this study, showing a pattern partially different from other reported virulence genes. Of the C. jejuni studied, 94.3%, 97.1% and 5.7% were positive for cadF, cdtB and cgtB, respectively. This study provides initial data on the prevalence and distribution of the flaA, cadF, cdtB, and cgtB genes in C. jejuni isolated from broiler meat in Serbia.

Keywords

slaughterhouse; C. jejuni; virulence genes

Hrčak ID:

235738

URI

https://hrcak.srce.hr/235738

Publication date:

16.3.2020.

Article data in other languages: croatian

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