Original scientific paper

Expression of Aspergillus oryzae Tannase in Pichia pastoris and Its Application in the Synthesis of Propyl Gallate in Organic Solvent

Xiao-Wei Yu ; Jiangnan University College of Biotechnology, CN-214122 Wuxi, PR China
Yong-Quan Li ; Zheijang University College of Life Science, CN-310058 Hangzhou, PR China

Abstract

Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of Aspergillus oryzae tannase was achieved in Pichia pastoris under the control of the AOX1 promoter, and the productivity of recombinant tannase was 960 U/L or 64 U/g dry mass. The recombinant P. pastoris was used to synthesize propyl gallate in organic solvent and the yield of propyl gallate was 53 %.

Keywords

tannase; Pichia pastoris; recombinant protein production; propyl gallate; biosynthesis

Hrčak ID:

22226

URI

https://hrcak.srce.hr/22226

Publication date:

14.3.2008.

Article data in other languages: croatian

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