Original scientific paper

Effect of silymarin and ochratoxin A on humoral natural immunity of broiler chickens

Stefan DENEV orcid.org/0000-0002-4849-8261 ; Department of Biochemistry, Microbiology and Physics, Agrarian Faculty, Thracian University, 6000 Stara Zagora, Bulgaria
Lilyan SOTIROV orcid.org/0000-0002-5967-497X ; Department of Animal Husbandry, Veterinary Faculty, Thracian University, 6000 Stara Zagora, Bulgaria
Sashka CHOBANOVA ; Department of Morphology, Physiology and Nutrition, Agrarian Faculty, Thracian University, 6000 Stara Zagora, Bulgaria
Tsvetoslav KOYNARSKI ; Department of Animal Husbandry, Veterinary Faculty, Thracian University, 6000 Stara Zagora, Bulgaria
Veselin IVANOV ; Trakia University, Medical Faculty, Department of Chemistry and Biochemistry, 6000 Stara Zagora, Bulgaria
Nadya BOZAKOVA ; Department of Animal Husbandry, Veterinary Faculty, Thracian University, 6000 Stara Zagora, Bulgaria
Stoicho STOEV ; Department of General and Clinical Pathology; Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria

Abstract

The aim of this work was to investigate the effect of Ochratoxin A (OTA) and Silymarin on serum lysozyme concentrations, complement and betalysin activity in broiler chickens. In this experiment 144 one-day-old Ross 308 male broiler chicks were used. All chicks were divided in four groups of 36 birds each: Group 1: Basal diet (BD) with no supplementation of Ochratoxin A (OTA) and Silymarin; Group 2: BD with 1.0% Silymarin; Group 3: BD with 3.0 mg/kg OTA; Group 4: BD with 3.0 mg/kg OTA plus 1.0% Silymarin. It was found that lysozyme concentration in the 2nd group there is a significant difference to groups 3 and 4. The immunosuppressive effect of Ochratoxin A is underlined but no protective effect of Silymarin in the group 4 was found. The alternative pathway of complement activation (APCA) is affected in the group 4. Betalysine there is a significant decreasing in the group 3 but slightly increasing of Betalysine in 4th group. Based on these results it can be concluded that OTA there is an immunosuppressive effect on the studied traits and there is a positive effect of Silymarin only on serum betalysine

Keywords

betalysine; chicken; complement; lysozyme; ochratoxin A; silymarin

Hrčak ID:

244296

URI

https://hrcak.srce.hr/244296

Publication date:

29.9.2020.

Article data in other languages: bulgarian

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