Professional paper

Validation parameters of instrumental method for determination of total bacterial count in milk

Nataša Mikulec ; Zavod za mljekarstvo, Agronomski fakultet, Svetošimunska 25, 10000 Zagreb
Dubravka Samaržija
Neven Antunac
Šimun Zamberlin
Zrinka Kuliš
Iva Horvat

Abstract

The method of flow citometry as rapid, instrumental and routine microbiological method is used for determination of total bacterial count in milk. The results of flow citometry are expressed as individual bacterial cells count. Problems regarding the interpretation of the results of total bacterial count can be avoided by transformation of the results of flow citometry method onto the scale of reference method (HRN ISO 6610:2001.). The method of flow citometry, like any analitycal method, according to the HRN EN ISO/IEC 17025:2000 standard, requires validation and verification. This paper describes parameters of validation: accuracy, precision, specificity, range, robustness and measuring uncertainty for the method of flow citometry.

Keywords

flow citometry; parameters of validation; total bacterial count

Hrčak ID:

1655

URI

https://hrcak.srce.hr/1655

Publication date:

10.10.2004.

Article data in other languages: croatian

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