Original scientific paper
Enzyme and Non-Enzyme Antioxidant Activity of Micro Propagated Cape Gooseberry (Physalis peruviana L.)
Maria Geneva
orcid.org/0000-0002-4014-0636
; Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, Sofia, Bulgaria
Ely Zayova
orcid.org/0000-0001-8754-2889
; Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, Sofia, Bulgaria
Ira Stancheva
; Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, Sofia, Bulgaria
Elisaveta Kirova
; Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, Sofia, Bulgaria
Mariana Sichanova
; Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Bldg. 21, Sofia, Bulgaria
Radhika Rathi
; Department of Biosciences, Manipal University Jaipur, Rajasthan, India
Mousumi Debnath
orcid.org/0000-0002-7094-466X
; Department of Biosciences, Manipal University Jaipur, Rajasthan, India
Abstract
Several studies have represented data on in vitro clonal multiplication of Physalis peruviana L. using different nutrient media supplemented with diverse growth regulators, but there is still scarce information on the changes in the antioxidant potential of the plants subjected to micropropagation. The present research investigates the effect of various plant growth regula-tors on the antioxidant activity of micropropagated P. peruviana L. The seedling explants of P. peruviana (L.) were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of the studied citokines, 6-benzylaminopurine (BAP), zeatin and thidiazuron (TDZ).The micro-shoots produced normal roots within two weeks of cultivation on half-strength MS medium supplemented with the auxins indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) at concentrations 0.1 mg L-1 and 0.5 mg L-1, respectively. The most efficient plant rooting was achieved with plants grown on half-strength MS medium with 0.1 mg L-1 IBA (number of shoots per explant 12.1 with a shoot height 5.2 cm) as well as 0.1 mg L-1 IAA (number of shoots per explant 10.4 with a shoot height 7.3 cm) after four weeks of cultivation. The reported micropropagation protocol here is characterized by a rapid proliferation and facilitated rooting of the microshoots. The multiple plants were successfully adapted showing 100% survival rate after two months of ex vitro growth. The changes of en-zyme and non-enzyme antioxidant activity were found to depend on the concentration of the used cytokinins. At the lower concentration of 0.5 mg L-1 of studied cytikinins the antioxidant capacity was characterized by a higher concentration of metabolites with antioxidant poten-tial. The higher cytokinin concentration of 0.1 mg L-1 used in the micropropagation procedure resulted in higher antioxidant enzyme activity. The influence of different growth regulators on the antioxidant potential of micropropagated P. peruviana L. plantlets is discussed.
Keywords
antioxidants, in vitro propagation, Physalis peruviana (L.), plant growth regulators
Hrčak ID:
287338
URI
Publication date:
15.12.2022.
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