Original scientific paper

https://doi.org/10.2478/aiht-2022-73-3667

Evaluation of bacterial uptake, antibacterial efficacy against Escherichia coli, and cytotoxic effects of moxifloxacin-loaded solid lipid nanoparticles

Merve Eylul Kiymaci orcid.org/0000-0001-5343-1064 ; University of Health Sciences Turkey, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Ankara, Turkey
Gizem Ruya Topal orcid.org/0000-0002-7715-6383 ; University of Health Sciences Turkey, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Ankara, Turkey
Ozgur Esim orcid.org/0000-0002-6402-156X ; University of Health Sciences Turkey, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Technology, Ankara, Turkey
Merve Bacanli orcid.org/0000-0001-8757-0572 ; University of Health Sciences Turkey, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey
Cansel Kose Ozkan orcid.org/0000-0002-4340-7279 ; University of Health Sciences Turkey, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Technology, Ankara, Turkey
Onur Erdem ; University of Health Sciences Turkey, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey
Ayhan Savaser ; University of Health Sciences Turkey, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Technology, Ankara, Turkey
Yalcin Ozkan ; University of Health Sciences Turkey, Gülhane Faculty of Pharmacy, Department of Pharmaceutical Technology, Ankara, Turkey

Abstract

Moxifloxacin (MOX) is an important antibiotic commonly used in the treatment of recurrent Escherichia coli (E. coli) infections. The aim of this study was to investigate its antibacterial efficiency when used with solid lipid nanoparticles (SNLs) and nanostructured lipid carriers (NLCs) as delivery vehicles. For this purpose we designed two SLNs (SLN1 and SLN2) and two NLCs (NLC1 and NLC2) of different characteristics (particle size, size distribution, zeta potential, and encapsulation efficiency) and loaded them with MOX to determine its release, antibacterial activity against E. coli, and their cytotoxicity to the RAW 264.7 monocyte/macrophage-like cell line in vitro. With bacterial uptake of 57.29 %, SLN1 turned out to be significantly more effective than MOX given as standard solution, whereas SLN2, NLC1, and NLC2 formulations with respective bacterial uptakes of 50.74 %, 39.26 %, and 32.79 %, showed similar activity to standard MOX. Cytotoxicity testing did not reveal significant toxicity of nanoparticles, whether MOX-free or MOX-loaded, against RAW 264.7 cells. Our findings may show the way for a development of effective lipid carriers that reduce side effects and increase antibacterial treatment efficacy in view of the growing antibiotic resistance.

Keywords

antibiotic; biocompatibility; drug resistance; E. coli; lipid-based nanoparticles; RAW 264.7 cell line

Hrčak ID:

288086

URI

https://hrcak.srce.hr/288086

Publication date:

28.12.2022.

Article data in other languages: croatian

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