Original scientific paper
https://doi.org/10.33128/k.64.2.2
The influence of propolis and bee pollen on the composition of the microbiological flora of a broiler’s crop
Ivana Prakatur
; Fakultet agrobiotehničkih znanosti Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska
Matija Domaćinović
; Fakultet agrobiotehničkih znanosti Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska
Mirela Pavić Vulinović
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Danijela Samac
orcid.org/0000-0001-9277-3710
; Fakultet agrobiotehničkih znanosti Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska
Abstract
The aim of this study was to determine the influence of the addition of propolis and/or bee pollen to broiler feed mixtures on the composition of the microbiological flora of a broiler’s crop. The study was conducted on a total of 200 one-dayold chickens of the Ross 308 hybrid, evenly distributed between the sexes, which were divided into five groups. During the entire experiment, the control group of chickens was fed with a feed mixture, while the experimental groups of chickens were fed with the same feed mixture with the addition of propolis and bee pollen, each additive separately or in combination in a certain ratio. Fattening was conducted on the wooden sawdust, and lasted for 42 days. From days 1-21 of the study chickens were fed a feed mixture of starter, and from days 22-42 of the study they were fed a finisher feed mixture. During the study, feed and water were given to chickens’ ad libitum. At the end of the study, on the 42nd day and after a 10-hour starvation, 10 chickens were randomly selected from each group and sacrificed. Samples of the broiler’s crop contents were taken in sterile vials, in which the total number of bacteria, the number of bacteria from the genus Enterobacteriaceae and the number of bacteria from the genus Lactobacillus were determined in an authorized microbiological laboratory. Microbiological analysis of the broiler’s crop contents showed that there were no statistically significant differences between the groups of chickens in the total number of bacteria and the number of bacteria from the genus Lactobacillus, while there were statistically significant differences in the number of bacteria from the genus Enterobacteriaceae (P=0.042) in the broiler’s crop content of the chickens of the control and experimental groups. The present study confirmed that the addition of propolis and/or bee pollen to the feed mixtures has a significantly positive effect on the occurrence of beneficial and pathogenic microorganisms in the contents of broiler’s crop, which was manifested by a statistically significantly lower number of bacteria from the genus Enterobacteriaceae in the broiler’s crop content of chickens of the experimental groups on the 42nd day of fattening compared to chickens of the control group.
Keywords
natural additives; propolis; bee pollen; broilers; broiler’s crop
Hrčak ID:
294622
URI
Publication date:
28.2.2023.
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