Original scientific paper

https://doi.org/10.24099/vet.arhiv.1862

Biološka karakterizacija izolata golubljeg paramiksovirusa 1 i analiza njegove patogenosti u SPF pilića

Yuxia Zhang ; Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Poultry Breeding Engineering Technology Center of Shandong Province, Jinan, China
Sufang Ren ; Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China
Xiaoyuan Yuan ; Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Poultry Breeding Engineering Technology Center of Shandong Province, Jinan, China
Jinxing Yang ; Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Poultry Breeding Engineering Technology Center of Shandong Province, Jinan, China
Wu Ai ; Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Poultry Breeding Engineering Technology Center of Shandong Province, Jinan, China
Kai Meng ; Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Poultry Breeding Engineering Technology Center of Shandong Province, Jinan, China

Abstract

Golublji paramiksovirus tipa 1 (PPMV-1) smatra se antigenskom varijantom njukaslske bolesti koja uzrokuje visoku smrtnost i znatne ekonomske gubitke u peradarskoj industriji. Patogenost virusa PPMV-1 u pilića međutim još uvijek nije jasna. U ovom je radu provedena biološka karakterizacija PPMV-1 izoliranog iz golubova za koje se sumnja da su oboljeli od njukaslske bolesti te je istražena njihova patogenost u SPF pilića. Filogenetsko stablo i evolucijske udaljenosti otkrili su da novi izolat pripada podgenotipu VI.2.1.1.2.2 virusa njukaslske bolesti (NDV). Unatoč motivu u mjestu cijepanja F-proteina koji sadržava sekvenciju 112RRQKR↓F117 povezanu s virulentnim sojevima NDV-a, vrijednosti MDT-a i ICPI-ja izolata pokazale su mezogene značajke. Ovo probno istraživanje je pokazalo da su izolati PPMV-1 slabo patogeni u pilića, dok u golubova uzrokuju lezije na više organa i tkiva.

Keywords

PPMV-1; podgenotip VI.2.1.1.2.2; genska karakterizacija; patogenost

Hrčak ID:

303106

URI

https://hrcak.srce.hr/303106

Publication date:

24.5.2023.

Article data in other languages: english

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