Original scientific paper

Investigation of different media content on meristem organization of raspberry (Rubus idaeus) in conditions in vitro

Elizabeta Angelova ; Faculty of Agriculture Science and Food, Skopje, R. Macedonia
Torso Arsov ; Faculty of Agriculture Science and Food, Skopje, R. Macedonia

Abstract

Optimal conditions for in vitro multiplication and rooting of cv. Willamette were investigated. High level of proliferation and multiplication after 90 days of culture on Murashige and Skoog (MS) medium with 100 mg·l-1 myo-inositol, 2 mg·l-1 thiamine-HCl, 1 mg·l-1 piridoxine-HCl, 1 mg·l-1 nicotinic acid and 30 g·l-1 sucrose and 8 g·l-1 agar was considered together with two concentrations of BA (0,2 and 2 mg·l-1). Half strength MS medium was also used. There was a significant variation of the proliferated shoots, depending on media. We got high level of rooting and we have investigated acclimatization of regenerants grown on sterile substrate in conditions of high humidity.

Keywords

raspberry; micropropagation in vitro; MS medium

Hrčak ID:

28155

URI

https://hrcak.srce.hr/28155

Publication date:

20.2.2007.

Article data in other languages: croatian

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