Original scientific paper
Indeksiranje i eliminacija virusa tristeza iz mandarine Unshiu (Citrus unshiu Marc.) cv. Kuno
Danijela Hartl-Musinov
; Institut za jadranske kulture i melioraciju krša, Split, Hrvatska
Katarina Hančević
J. Rošin
Ž. Gatin
Abstract
The main grown citrus crop in Croatia is Satsuma mandarin (Citrus unshiu Marc.). The preliminary surveys have shown widespread occurrence of the most devastating citrus pathogen, Citrus tristeza virus (CTV) in Croatia. In this research four Satsuma mandarin cultivars: Chahara, Ichimaru, Kuno and Zorica Rana were biologically tested for the presence of CTV on Mexican lime (Citrus aurantifolia Swingle) bioindicator. Displayed symptoms strongly suggest the existence of mild CTV strains in four tested mandarin cultivars. Laboratory diagnostic methods (DTBIA, DAS-ELISA, IC/RT-PCR) confirmed these findings. Plants of cv. Kuno mandarin underwent sanitation treatments which consisted of thermotherapy and shoot-tip grafting (STG; micrografting) on in vitro germinated Troyer citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck)] rootstock. Tristeza-virus was eliminated from 42% of infected ’Kuno’ mandarins (confirmed by DTBIA). Completion of the above mentioned procedures will help development and implementation of citrus certification program in Croatia which would ensure the production of high-quality citrus propagating material in both sanitary and pomological aspects.
Keywords
bioindicator; micrografting; certification
Hrčak ID:
28157
URI
Publication date:
20.2.2007.
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