Original scientific paper

Pharmacokinetics after single intramuscular administration and in vitro plasma protein binding of cefoperazone in cross bred calves

Jiwan Kumar Gupta ; Department of Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Rakesh Kumar Chaudhary ; Department of Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Vinod Kumar Dumka ; Department of Pharmacology and Toxicology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India

Abstract

The present study was conducted to investigate the pharmacokinetics of cefoperazone after single intramuscular (i/m) administration of 20 mg.kg-1 into the lateral neck region and its in vitro plasma protein binding in male cross bred calves. The concentration of cefoperazone in plasma samples was estimated by a standard microbiological assay technique using Escherichia coli (ATCC 10536) as the test organism. Appreciable plasma concentration of cefoperazone (1.14 ± 0.07 μg.mL-1) was detected at 1 min after injection and the peak plasma level of 9.76 ± 0.25 μg.mL-1 was observed at 45 min. The drug level above MIC90 in
plasma, was detected up to 5 h of administration. Rapid absorption of the drug was also evident by the short absorption half-life (0.55 ± 0.08 h). The overall systemic bioavailability of cefoperazone after intramuscular administration was 48.1 ± 5.33%. The high value of AUC (15.7 ± 0.64 μg.mL-1.h) reflected a vast area of body covered by drug concentration. Extensive distribution of the drug into various body fluids and tissues was reflected by the high value of the steady state volume of distribution (2.95 ± 0.28 L.kg-1). The elimination halflife and MRT were 2.31 ± 0.05 h and 3.62 ± 0.06 h, respectively. The total body clearance (ClB) was 1.28 ± 0.05 L.kg-1.h-1. Cefoperazone was bound to the plasma proteins of calves to the extent of 24.9 ± 1.11%. There was no statistically significant difference in the pharmacokinetic parameters calculated by compartmental and non-compartmental analysis except the values of AUC, AUMC and ClB. On the basis of the pharmacokinetic parameters, an appropriate i/m dosage regimen for cefoperazone in calves would be 26 mg. kg-1 followed by 22 mg.kg-1 at 6 h intervals.

Keywords

calves; cefoperazone; pharmacokinetics; protein binding

Hrčak ID:

28920

URI

https://hrcak.srce.hr/28920

Publication date:

20.10.2008.

Article data in other languages: croatian

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