Acta Pharmaceutica, Vol. 74 No. 3, 2024.
Original scientific paper
https://doi.org/10.2478/acph-2024-0020
Tannic acid elicits differential gene regulation in prostate cancer apoptosis
SINAN KANDIR
; Faculty of Veterinary Medicine, Department of Physiology, Çukurova University, 01920 Ceyhan, Adana, Turkiye
SEVTAP KARAKURT
; Faculty of Science, Department of Biochemistry, Selçuk University, 42250 Konya, Turkiye
ÇIĞDEM GÖKÇEK-SARAÇ
; Faculty of Engineering, Department of Biomedical Engineering, Akdeniz University, 07050 Antalya, Turkiye
*
SERDAR KARAKURT
; Faculty of Science, Department of Biochemistry, Selçuk University, 42250 Konya, Turkiye
* Corresponding author.
Abstract
Prostate cancer is a significant global health concern that requires innovative therapeutic investigations. Here, the potential anti-cancer properties of tannic acid were evaluated by examining its effects on apoptosis in prostate cancer cell lines. PC-3 and LnCaP prostate adenocarcinoma cells, along with PNT1A prostate control cells, were cultured and divided into untreated and tannic acid-treated groups. Cell proliferation, cytotoxicity, and effects of tannic acid on the cell death mechanism were evaluated. mRNA expression levels of 84 genes were explored in cells following tannic acid treatment. Notably, tannic acid-induced down-regulation of several pro-survival genes, including ATM, BCL2, BCL2A1, BIK, BIRC2, BIRC3, BRE, CASP3, CASP6, CASP8, CHEK2, CRADD, PPIA, RPA3, TNFSF18, TRAF1, TRAF2, TRAF4, and TRAF5 in both cell lines. Moreover, tannic acid treatment led to the up-regulation of various pro-apoptotic genes, such as BCL10, BIRC3, BNIP3, CASP1, CASP5, CD40, CIDEB, DAPK2, FASLG, GADD45A, MYD88, RPA3, TNFRSF10D, TNFRSF17, TNFRSF8, TNFSF13B, TNFSF4, TNFSF7, TNFSF8, TNFSF9, TP53, TRAF1, and TRAF2 in both PC-3 and LnCap cells. These findings highlight tannic acid's ability to induce apoptosis in prostate cancer cells through pro-apoptotic pathways. This study concludes that tannic acid selectively inhibits prostate cancer cell growth.
Keywords
tannic acid; prostate cancer; apoptosis; gene regulation; selective cytotoxicity
Hrčak ID:
317611
URI
Publication date:
30.9.2024.
Visits: 0 *