Original scientific paper
Optimization of Media for Enhanced Glucoamylase Production in Solid-State Fermentation by Fusarium solani
Haq Nawaz Bhatti
; Department of Chemistry, University of Agriculture, 38040 Faisalabad, Pakistan
Mohammad Hamid Rashid
; National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, 38000 Faisalabad, Pakistan
Rakhshanda Nawaz
; Department of Chemistry, University of Agriculture, 38040 Faisalabad, Pakistan
Muhammad Asgher
; Department of Chemistry, University of Agriculture, 38040 Faisalabad, Pakistan
Raheela Perveen
; National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, 38000 Faisalabad, Pakistan
Abdul Jabbar
; Department of Chemistry, Government College University, 38000 Faisalabad, Pakistan
Abstract
Solid-state cultivation of Fusarium solani was carried out for enhanced production of glucoamylase (GA) using different substrates like wheat bran, rice bran, green gram bran, black gram bran and maize bran. The SSF medium containing wheat bran as a substrate yielded the highest enzyme activity. The physical and chemical parameters were optimized. Maximum enzyme activity (61.35±3.69) U/g of dry wheat bran was achieved under optimum growth conditions. The optimum conditions were fructose as carbon and energy additive 1 % (by mass), urea as nitrogen additive 1 % (by mass), initial moisture content of solid substrate 70 % (by mass per volume), incubation period 96 h, inoculum size 15 % (by mass per volume) having 10^6–10^7 spores/mL, incubation temperature (35±1) °C and pH=5.0. It was further observed that the addition of surfactants caused a decrease in enzyme biosynthesis by F. solani in SSF of wheat bran under optimum process conditions.
Keywords
glucoamylase; wheat bran; optimization; solid-state fermentation; moisture content
Hrčak ID:
30436
URI
Publication date:
15.3.2007.
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