Preliminary communication

Antioxidative Activity of Tobacco Leaf Protein Hydrolysates

Guohua Rao ; College of Light Industry and Food, South China University of Technology (SCUT), 510640 Guangzhou, PR China
Mouming Zhao ; College of Light Industry and Food, South China University of Technology (SCUT), 510640 Guangzhou, PR China
Weifeng Lin ; College of Light Industry and Food, South China University of Technology (SCUT), 510640 Guangzhou, PR China
Haiyan Wang ; College of Light Industry and Food, South China University of Technology (SCUT), 510640 Guangzhou, PR China

Abstract

Discarded tobacco leaf protein hydrolysate (DTLPH) was prepared by enzymatic hydrolysis using papain and then separated using ultrafiltration (UF) membranes with molecular mass cut-off (MMCO) of 10, 5, 3 and 1 kDa. Four permeate fractions including 10-K, 5-K, 3-K and 1-K (the permeate fractions from 10, 5, 3 and 1 kDa) hydrolysate fractions were obtained. The 5-K hydrolysate fraction had high oxidation inhibilitory ratio (42.62 %), which was about twofold higher than the original hydrolysate and as high as that of vitamin E (α-tocopherol). The fractionated hydrolysates were superior to the original hydrolysate in the antioxidative activity tested. Moreover, these separated hydrolysates showed the enhanced functional property. The amino acid composition of 5-K hydrolysate was analyzed and the results show that the high antioxidative activity of 5-K hydrolysate was derived from high content of histidine, methionine, cystine and tryptophan.

Keywords

discarded tobacco leaf protein; enzymatic hydrolysate; ultrafiltration membrane; antioxidative activity; amino acid composition

Hrčak ID:

30440

URI

https://hrcak.srce.hr/30440

Publication date:

15.3.2007.

Article data in other languages: croatian

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