Original scientific paper
Investigation of Genomic Instability in Patients with Sjögren’s Syndrome by Using Sister Chromatid Exchange Analysis
Sertan Ergun
; Istanbul University, Faculty of Dentistry, Department of Oral Medicine and Surgery, Istanbul, Turkey
Hakki Tanyeri
; Istanbul University, Faculty of Dentistry, Department of Oral Medicine and Surgery, Istanbul, Turkey
Şükrü Öztürk
; Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Medical Genetics, Istanbul, Turkeya
Nilgün Duman
; Istanbul University, Institute of Health Science, Department of Genetics, Istanbul, Turkey
Sevil Kamal
; Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Rheumatology, Istanbul, Turkey
Ahmet Gül
; Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Rheumatology, Istanbul, Turkey
Reyhan Küçükkaya
; Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Hematology, Istanbul, Turkey
Sevda Özel
; Istanbul University, Istanbul Faculty of Medicine, Department of Public Health, Istanbul, Turkey
Kivanç Çefle
; Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Medical Genetics, Istanbul, Turkey
Sükrü Palanduz
; Istanbul University, Istanbul Faculty of Medicine, Department of Internal Medicine, Division of Medical Genetics, Istanbul, Turkeya
Abstract
Purpose: To investigate genomic instability by using Sister Chromatid Exchange (SCE) in the peripheral blood lymphocytes in patients with Sjögren’s syndrome (SS). Patients and Methods: The frequency of SCE (per metaphase) was investigated in patients with primary Sjögren’s Syndrome (n=30), in untreated lymphoma patients (n=15) and healthy individuals (n=15) who constitute the control groups. Results: We found the mean frequencies of SCE in the patients with SS, untreated lymphoma and healthy controls as 7.77±1.50, 8.80±0.75, 6.65±1.50, respectively. We found statistically significant differences between patients with SS and lymphoma (p=0.007) and also between patients with lymphoma and healthy controls (p=0.0001). No significant difference was observed between patients with SS and healthy controls in regard of mean frequencies of SCE (per metaphase). Conclusion: It is well known that up to 5.0% of all SS patients may develop lymphoma. High frequency of SCE is regarded as a cytogenetic biomarker of early mutagenic effect suggesting increased risk of cancer. We observed that the mean frequencies of sister chromatid exchanges (per metaphase) of three patients with SS were higher than those of untreated lymphoma patients. This result can only be verified by prolonged follow-up of these patients. We suggest that results should be interpreted by evaluating other parameters which play important roles in malignant transformation.
Keywords
Sjögren’s syndrome; sister chromatid exchange; lymphoma
Hrčak ID:
30519
URI
Publication date:
11.12.2008.
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