Croatica Chemica Acta, Vol. 81 No. 4, 2008.
Original scientific paper
Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products
Biljana Nigović
; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Ivana Vegar
; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
Abstract
A capillary zone electrophoresis method for pravastatin determination was developed and validated.
Rapid migration of negatively charged pravastatin molecule was obtained in alkaline
buffer by the application of electric field of 30 kV. Influence of the pH value and ionic strength
of running buffer, applied voltage and capillary temperature on mobility and sensitivity was
evaluated. Detection wavelength was set to 237 nm. The method was applied to the determination
of the drug in pharmaceutical dosage form. Pravastatin is a δ-hydroxy acid, which is prone
to lactonize and epimerize in a pH-dependent manner. Micellar electrokinetic chromatographic
approach was chosen to develop a method able to separate pravastatin and its degradation
products in acidic media. The proposed method allows baseline separation of hydroxy acid and
neutral lactone forms of the drug that appear as interconversion products depending on the pH
value.
Keywords
pravastatin; capillary zone electrophoresis; pharmaceuticals; micellar electrokinetic chromatography; degradation products
Hrčak ID:
31187
URI
Publication date:
31.12.2008.
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