Original scientific paper
https://doi.org/https://doi.org/10.5513/JCEA01/27.3.5109
Antifungal activity of Bacillus amyloliquefaciens filtrate against Fusarium dry rot of potato
Katarina MARTINKO
; University of Zagreb Faculty of Agriculture, Division of Phytomedicine, Department of Plant Pathology, Svetošimunska 25, 10000 Zagreb, Croatia
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* Corresponding author.
Abstract
Potato (Solanum tuberosum) tuber dry rot caused by Fusarium spp. leads to substantial losses during storage and may be exacerbated by secondary soft-rot bacteria. Due to limitations of chemical control, Bacillus-based biocontrol is increasingly explored as a sustainable alternative. This study evaluated the antagonistic potential of Bacillus amyloliquefaciens culture filtrate against Fusarium oxysporum and F. graminearum. In vitro poisoned food assay with 1% (v/v) filtrate showed strong inhibition of mycelial growth, reaching 93% for F. oxysporum and 91% for F. graminearum after 7 days compared to the control. In vivo preventive treatment of wounded potato tubers significantly reduced dry rot development after 21 days, by 72% (F. oxysporum) and 84% (F. graminearum). Microscopic observations revealed pronounced hyphal abnormalities in treated variants (hyphal swelling, vacuolisation and increased chlamydospore formation), indicating severe pathogen stress. Overall, the results demonstrate that B. amyloliquefaciens filtrate is a highly effective biocontrol agent against potato Fusarium dry rot, with potential for inclusion in sustainable postharvest disease management.
Keywords
potato; Bacillus amyloliquefaciens; Fusarium oxysporum; Fusarium graminearum; biological control
Hrčak ID:
351492
URI
Publication date:
1.10.2026.
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