Biochemia Medica, Vol. 19 No. 1, 2009.
Professional paper
Sperm morphology assessment according to WHO and strict criteria: method comparison and intra-laboratory variability
Andrea Čipak
; University Department of Obstetrics and Gynecology, Zagreb University Hospital Center, Zagreb, Croatia
Patrik Stanić
; University Department of Obstetrics and Gynecology, Zagreb University Hospital Center, Zagreb, Croatia
Koraljka Đurić
; Medical Biochemistry Laboratory, Sunce Polyclinic, Zagreb, Croatia
Tihana Serdar
; Department of Laboratory Medicine, Dubrava University Hospital, Zagreb, Croatia
Ernest Suchanek
; Fertility Clinic,Tawam Hospital, Johns Hopkins Medicine, Al Ain, Abu Dhabi, U.A.E.
Abstract
Background: Assessment of sperm morphology is one of the most important steps in the evaluation of male partner in infertile couples. However, significant inter- and intra-laboratory variations can cause difficulties in interpretation, misdiagnoses, and consequently lead to confusion. Therefore, it is necessary to minimize these variations to eliminate consequential errors and ensure intra- and inter-laboratory reproducibility.
Materials and methods: Semen specimens were obtained from 49 consecutive male patients attending Andrology Laboratory for fertility evaluation. Two sperm morphology assessment criteria were compared: 1) semen smear prepared by Giemsa and assessed by World Health Organization (WHO) criteria; and 2) semen smear prepared by Spermac and assessed by strict criteria. Intra-laboratory comparison of morphological examination was also carried out.
Results: The diagnosis of teratozoospermia by both WHO and strict criteria was concordant in 45 of 49 cases. Intra-rater agreement between the observers was similar for WHO and strict criteria (kappa, 0.700 vs. 0.715).
Conclusions: Morphology assessment by WHO and strict criteria is concordant in diagnosing teratozoospermia and good inter-observer agreement can be achieved after proper training, mindful smear examination and complying with the classification systems.
Keywords
spermatozoa; infertility; male; observer variation
Hrčak ID:
32268
URI
Publication date:
5.2.2009.
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