Original scientific paper

Ochratoxin A Induces Apoptotic and Necrotic Renal Cell Death

József Petrik
Ana Malić
Karmela Barišić
Lada Rumora
Tamás Kõszegi
Stjepan Pepeljnjak
Tihana Žanić-Grubišić
Ivana Čepelak

Abstract

MDCK, LLC-PK1 and RK 13 renal cells were exposed to different OTA concentrations (2.5 to 25 μg OTA/mL of medium) for 24 hours. 2.5 μg OTA/mL significantly reduced the number of living RK 13 cells to 69.4 %, LLC-PK1 cells to 34.5 % and MDCK cells to 27.5 %, as compared to untreated control cells. Effects of OTA on cell morphology were examined by haematoxylin/ eosin or phalloidin-FITC/propidium iodide staining. 12 %, 11 % and 8.2 % apoptotic cells were observed in MDCK, LLC-PK1 and RK 13 cells treated with 2.5 μg OTA/mL, respectively. The structure of actin cytoskeleton showed significant changes in OTA-treated cells. Release of LDH into the culture medium was more abundant in MDCK and LLC-PK1 cells. OTA induced apoptotic and/or necrotic cell death in a cell type- and concentration-dependent manner. The results suggest that MDCK and LLC-PK1 cells are more sensitive to OTA than RK 13 cells.

Keywords

ochratoxin A; apoptosis; necrosis; MDCK cells; LLC-PK1 cells; RK 13 cells

Hrčak ID:

56

URI

https://hrcak.srce.hr/56

Publication date:

15.9.2005.

Article data in other languages: croatian

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