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Food Technology and Biotechnology, Vol. 47 No. 2, 2009.

Original scientific paper

Sinteza sukcinske kiseline s pomoću kvasaca uzgojenih na podlozi s etanolom

Svetlana V. Kamzolova orcid id orcid.org/0000-0003-4326-2345 ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia
Alsu I. Yusupova ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia
Emiliya G. Dedyukhina ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia
Tatiana I. Chistyakova ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia
Tatiana M. Kozyreva ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia
Igor G. Morgunov ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia

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Abstract

Ispitana je sinteza sukcinske kiseline s pomoću 32 vrste kvasaca različitih rodova (Debaryomyces, Candida, Pichia, Saccharomyces i Torulopsis), uzgojenih na podlozi s etanolom. Otkriveno je da 29 vrsta ima sposobnost sinteze sukcinske kiseline, od kojih su izdvojene dvije najučinkovitije. Uzgojem na podlozi s mineralnim tvarima, u bioreaktoru s pojačanom aeracijom uz dodatak etanola, soj Candida catenulata VKM Y-5 proizveo je 5,2 g/L sukcinske kiseline, s masenim iskorištenjem od 32,6 % i energetskim od 14,8 %, a soj Candida zeylanoides VKM Y-2324 izlučio je 9,4 g/L sukcinske kiseline, s masenim iskorištenjem od 39 % i energetskim od 17,8 %. Utvrđeno je da uz sukcinsku kiselinu nastaje znatna količina malonske kiseline, vjerojatno zbog veće aktivnosti enzima koji sudjeluju u glioksilatnom ciklusu. Ispitane su značajke rasta oba soja ovisno o koncentraciji etanola, cinkovih iona i dušika u podlozi.

Keywords

sukcinska kiselina; Candida catenulata; Candida zeylanoides; etanol

Hrčak ID:

38104

URI

https://hrcak.srce.hr/38104

Publication date:

9.6.2009.

Article data in other languages: english

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