Preliminary communication

Isolation of the Oleaginous Yeasts from the Soil and Studies of Their Lipid-Producing Capacities

Li-Xia Pan ; College of Life Science and Technology, Guangxi University, CN-530004 Nanning, PR China
Deng-Feng Yang ; Guangxi Academy of Sciences, CN-530003 Nanning, PR China
Li Shao ; College of Life Science and Technology, Guangxi University, CN-530004 Nanning, PR China
Wei Li ; College of Life Science and Technology, Guangxi University, CN-530004 Nanning, PR China
Gui-Guang Chen ; College of Life Science and Technology, Guangxi University, CN-530004 Nanning, PR China
Zhi-Qun Liang ; College of Life Science and Technology, Guangxi University, CN-530004 Nanning, PR China

Abstract

D-xylose is one of the most abundant pentose sugars in nature. To isolate oleaginous yeasts that can utilize xylose from diverse soil samples, soils from a litchi orchard, longan orchard, carambola orchard, and woods were collected, yeasts were isolated by the glycerol enrichment and their xylose-assimilating capacities were measured. A subset of these isolates was grown in nitrogen-limited media and then screened for potential oleaginous yeasts by the Sudan Black B staining, after which their lipid-producing capacities were studied. There were 13 strains of oleaginous yeasts identified, and a rapid microbiological assay was provided to exploit microbial lipids that may one day be used as biodiesels or cocoa butter substitute.

Keywords

isolation and identification of oleaginous yeast; xylose; glycerol; biodiesel; cocoa butter substitute

Hrčak ID:

38116

URI

https://hrcak.srce.hr/38116

Publication date:

9.6.2009.

Article data in other languages: croatian

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