Original scientific paper
Control of Some Human Pathogenic Bacteria by Seed Extracts of Cumin (Cuminum cyminum L.)
Mominul Islam Sheikh
; Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh; Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh
Soriful Islam
; Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
Atikur Rahman
; Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
Mostafizur Rahman
; Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
Mashiur Rahman
; Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh
Mizanur Rahman
; Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh
Abdur Rahim
; Department of Genetics and Plant Breeding, Sher-e Bangla Agricultural University, Dhaka-1207, Bangladesh
Firoz Alam
; Biotechnology and Microbiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh
Abstract
Antibacterial activity of seed extracts of cumin (Cuminum cyminum L.) was investigated against 10 gram positive and gram negative bacteria. Disc diffusion method was used to test antibacterial activity. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by using standard procedures. The highest (effective) inhibition zone of 16.67±0.47 mm was found at 250 mg/ml for Escherichia coli. On the other hand, the inhibition zones 15.00±0.82 mm for ethanol, 15.33±0.47 for methanol, and 15.67±0.82 for acetone were found against Bacillus subtilis, Sarcina lutea and Klebsiella pneumonia, respectively. MIC value (20 to 50 mg/ml) and MBC value (40 to 60 mg/ml) were measured against studied bacteria. On the basis of investigation, we can say, cumin seeds could be used as a source of new antibacterial agent for developing drugs to inhibit some human pathogenic bacteria.
Keywords
Cumin seed; antibacterial activity; MIC; MBC
Hrčak ID:
53007
URI
Publication date:
26.3.2010.
Visits: 1.569 *