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Agriculturae Conspectus Scientificus, Vol. 75 No. 3, 2010.

Preliminary communication

ESEM Comparative Studies of Hop (Humulus lupulus L.) Peltate and Bulbous Glandular Trichomes Structure

Siniša Srečec orcid id orcid.org/0000-0002-9009-4375 ; Križevci College of Agriculture, M. Demerca 1, HR-48260 Križevci, Croatia
Vesna Zechner-Krpan ; University of Zagreb, Faculty of Food Technology and Biotechnology,Department of Biochemical Engineering, Pierottijeva 6, HR-10000 Zagreb, Croatia
Vlatka Petravić-Tominac orcid id orcid.org/0000-0002-0680-5702 ; University of Zagreb, Faculty of Food Technology and Biotechnology,Department of Biochemical Engineering, Pierottijeva 6, HR-10000 Zagreb, Croatia
Gordan Mršić ; Forensic Science Centre Ivan Vučetić, Ilica 335, HR-10000 Zagreb, Croatia
Igor Špoljarić ; Forensic Science Centre Ivan Vučetić, Ilica 335, HR-10000 Zagreb, Croatia
Sanja Marag ; University of Zagreb, Faculty of Food Technology and Biotechnology,Department of Biochemical Engineering, Pierottijeva 6, HR-10000 Zagreb, Croatia

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Abstract

Hop glandular trichomes are developing on the epidermis of hop cones bracts. The anticline cross cut of hop peltate glandular trichomes in Environment Scanning Electron Microscopy (ESEM) observations confirmed spheroid shape of cells. In our research, length of internal peltate glandular trichomes cells varied from 16.00 to 21.47 μm, width from 4.21 to 4.70 μm and volume from 1.19 × 10-6 to 1.99 × 10-6 mm3. The diameter of cell walls varied from 2.11 to 2.94 μm. Volume of observed peltate glandular trichomes varied from 1.65 × 10-2 to 1.95 × 10-2 mm3. The cell structure of bulbous glandular trichomes in anticline cross cut was not observed. According to 121 observations, bulbs formation was visible on the surface of peltate glandular trichomes in the last phase of morphogenesis. Biosynthesis of hop secondary metabolites and activation of their genetic mechanisms are the most intensive at the end of technological maturity of hop cones of hop cultivar Aurora. It is possible that bulbous glandular trichomes represent the final stage of peltate glandular trichome morphogenesis, instead of being separate morphological formations. During biosynthesis of hops secondary metabolites partial pressures of liquids and gasses increase causing breakage of cell walls, which withdraw towards tunica and form papillary texture.

Keywords

hop; Humulus lupulus; ESEM; peltate glandular trichomes; bulbous glandular trichomes; biosynthesis of hop secondary metabolites

Hrčak ID:

65202

URI

https://hrcak.srce.hr/65202

Publication date:

22.9.2010.

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