Preliminary communication
Extracts from Fermented Black Soybean Milk Exhibit Antioxidant and Cytotoxic Activities
Kuan-Chen Cheng
; Institute of Microbiology and Biochemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 106 Taipei, Taiwan, ROC
Jiun-Tsai Lin
; Institute of Microbiology and Biochemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 106 Taipei, Taiwan, ROC
Wen-Hsiung Liu
; Institute of Microbiology and Biochemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 106 Taipei, Taiwan, ROC
Abstract
In this study, ethanol extracts from 2-day fermented black soybean milk (FBE) by immobilized Rhizopus oligosporus NTU5 have been evaluated for both antioxidant and cytotoxic activities. The results reveal that a 2-day FBE had strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging effect (76 %). The extracts were further fractionated by silica gel column chromatography and an unknown compound, FBE5-A, was obtained, which exhibited strong antioxidant activity. IC50 of the DPPH scavenging effect of FBE5-A was 7.5 μg/mL, which is stronger than a commonly used antioxidant, vitamin E (α-tocopherol; 17.4 μg/mL), and similar to vitamin C (ascorbic acid; 7.6 μg/mL). The cytotoxic test demonstrated that extracts of 2-day fermented broth exhibited selective cytotoxic activity towards human carcinoma cells, Hep 3B (IC50=150.2 μg/mL), and did not affect normal human lung fibroblasts, MRC-5 (p<0.05). The results indicate the potential applications of fermented black soybean milk as functional food, pharmaceutical or cancer therapy formula.
Keywords
black soybean; Rhizopus oligosporous; antioxidant activity; cytotoxic activity; isoflavone
Hrčak ID:
65628
URI
Publication date:
21.3.2011.
Visits: 2.747 *