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Arsenic trioxide treatment of rabbit liver VX-2 carcinoma via hepatic arterial cannulationinduced apoptosis and decreased levels of survivin in the tumor tissue
Hong Li
; Department of Radiology, First Affiliated Hospital of China Medical University, Shenyang, P. R. China
Jian Gong
; Department of Clinical Pharma cy School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, P. R. China
Xuyuan Jiang
; Department of Radiology, First Affiliated Hospital of China Medical University, Shenyang, P. R. China
Haibo Shao
; Department of Radiology, First Affiliated Hospital of China Medical University, Shenyang, P. R. China
Sažetak
Aim To investigate the role of tumor apoptosis-inhibitory
protein survivin in arsenic trioxide-induced apoptosis in
VX-2 carcinoma in the rabbit liver by means of transcatheter
arterial chemoembolization.
Methods Sixteen rabbits with 32 implanted hepatic VX-2
tumors were randomly divided into two groups. The experimental
group received 2 mg of arsenic trioxide and
1 mL of ultra-fluid lipiodol co-injected via hepatic arterial
cannulation and the control group received only 1 mL of
lipiodol. Animals were sacrificed 3 weeks after trans-catheterial
arterial chemoembolization. Tumor tissue and tumor-
peripheral tissue were collected for analysis. Terminal
deoxynucleotidyl transferase-mediated dUTP nick-end-labeling
staining was used to assess tumor cells apoptosis.
Immunohistochemistry was used to assess the presence
of survivin protein. Reverse transcription polymerase chain
reaction was used to determine the expression of survivin
gene.
Results The number of apoptotic cells significantly increased
in the tumor tissue (5.20 ± 0.60%) compared to
tumor-peripheral tissue (1.29 ± 0.42%) of the arsenic trioxide-
treated group. Survivin expression levels in the tumor
tissue were significantly reduced in arsenic trioxidetreated
group (7.68 ± 0.65) compared to the control group
(35.30 ± 4.63).
Conclusion Transcatheter arterial chemoembolization
with arsenic trioxide induced apoptosis of VX-2 carcinoma,
in which tumor apoptosis-inhibitory protein survivin may
have played a role.
Ključne riječi
Hrčak ID:
102768
URI
Datum izdavanja:
15.2.2013.
Posjeta: 1.311 *