Izvorni znanstveni članak

https://doi.org/10.3325/cmj.2014.55.259

Detection of novel autoantigens in patients with recurrent miscarriage: description of an approach and preliminary findings

Yuriy Kit ; Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
Marina Starykovych ; Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
Marie Vajrychova ; Faculty of Military Health Sciences, Hradec Kralove, Czech Republic
Juraj Lenco ; Faculty of Military Health Sciences, Hradec Kralove, Czech Republic
Danuta Zastavna ; Institute of Hereditary Pathology, National Academy of Medical Sciences of Ukraine, Lviv, Ukraine
Rostyslav Stoika ; Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine

Sažetak

Aim To develop and test a protocol for isolation of potential
auto-antigens from chorionic tissue that may be linked
to recurrent miscarriage (RM).
Methods The strategy included: 1) isolation of IgGs tightly
bound to chorionic tissue of RM patients by protein G
chromatography; 2) construction of affinity columns using
the chorionic antibodies for isolation of auto-antigens; 3)
enrichment of auto-antigens from detergent extracted solution
of chorionic proteins by affinity chromatography; 4)
separation by dodecyl sulfate-electrophoresis followed by
matrix-assisted laser desorption/ionization-time of flight
mass spectrometry identification.
Results Five potential auto-antigens were detected: neutral
alpha-glucosidase AB, endoplasmin, transitional endoplasmic
reticulum ATPase, putative endoplasmin-like protein,
and cytoplasmic actin 2.
Conclusions We developed a strategy for identification of
auto-antigens in the chorionic tissue of women with RM,
which could be of diagnostic and prognostic value.

Ključne riječi

Hrčak ID:

129842

URI

https://hrcak.srce.hr/129842

Datum izdavanja:

15.6.2014.

Posjeta: 1.167 *