Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2015.56.447
In female rats, ethylene glycol treatment elevates protein expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) without inducing hyperoxaluria
Davorka Breljak
; Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Hrvoje Brzica
; Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ivana Vrhovac
; Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Vedran Micek
; Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Dean Karaica
; Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Marija Ljubojević
; Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ankica Sekovanić
; Metabolism, Institute for Medical Research and Occupational Health, Zagreb, CroatiaAnalytical Toxicology & Mineral
Jasna Jurasović
; Metabolism, Institute for Medical Research and Occupational Health, Zagreb, CroatiaAnalytical Toxicology & Mineral
Dubravka Rašić
; Toxicology Units, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Maja Peraica
; Toxicology Units, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Mila Lovrić
; Clinical Institute of Laboratory Diagnosis, University HospitalCenter, Zagreb, Croatia
Nina Schnedler
; Systemic Physiology and Pathophysiology, University Medical Center Göttingen Göttingen, Germany
Maja Henjakovic
; Systemic Physiology and Pathophysiology, University Medical Center Göttingen Göttingen, Germany
Waja Wegner
; Systemic Physiology and Pathophysiology, University Medical Center Göttingen Göttingen, Germany
Gerhard Burckhardt
; Systemic Physiology and Pathophysiology, University Medical Center Göttingen Göttingen, Germany
Birgitta C. Burckhardt
; Systemic Physiology and Pathophysiology, University Medical Center Göttingen Göttingen, Germany
Ivan Sabolić
orcid.org/0000-0002-2587-9109
; Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Sažetak
Aim To investigate whether the sex-dependent expression
of hepatic and renal oxalate transporter sat-1 (Slc26a1)
changes in a rat model of ethylene glycol (EG)-induced hyperoxaluria.
Methods Rats were given tap water (12 males and 12 females;
controls) or EG (12 males and 12 females; 0.75%
v/v in tap water) for one month. Oxaluric state was confirmed
by biochemical parameters in blood plasma, urine,
and tissues. Expression of sat-1 and rate-limiting enzymes
of oxalate synthesis, alcohol dehydrogenase 1 (Adh1) and
hydroxy-acid oxidase 1 (Hao1), was determined by immunocytochemistry
(protein) and/or real time reverse transcription
polymerase chain reaction (mRNA).
Results EG-treated males had significantly higher (in
μmol/L; mean ± standard deviation) plasma (59.7 ± 27.2 vs
12.9 ± 4.1, P < 0.001) and urine (3716 ± 1726 vs 241 ± 204,
P < 0.001) oxalate levels, and more abundant oxalate
crystaluria than controls, while the liver and kidney sat-1
protein and mRNA expression did not differ significantly
between these groups. EG-treated females, in comparison
with controls had significantly higher (in μmol/L) serum
oxalate levels (18.8 ± 2.9 vs 11.6 ± 4.9, P < 0.001), unchanged
urine oxalate levels, low oxalate crystaluria, and
significantly higher expression (in relative fluorescence
units) of the liver (1.59 ± 0.61 vs 0.56 ± 0.39, P = 0.006) and
kidney (1.77 ± 0.42 vs 0.69 ± 0.27, P < 0.001) sat-1 protein,
but not mRNA. The mRNA expression of Adh1 was femaledominant
and that of Hao1 male-dominant, but both were
unaffected by EG treatment.
Conclusions An increased expression of hepatic and renal
oxalate transporting protein sat-1 in EG-treated female rats
could protect from hyperoxaluria and oxalate urolithiasis.
Ključne riječi
Hrčak ID:
151738
URI
Datum izdavanja:
15.10.2015.
Posjeta: 1.483 *