Izvorni znanstveni članak

https://doi.org/10.18054/pb.2016.118.1.3771

Detection of virulence gene belonging to cag pathogenicity island in Helicobacter pylori isolates after multiple unsuccessful eradication therapy in Northwest Croatia

Dijana Varda Brkić orcid.org/0000-0001-5614-9486 ; Department of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, Kišpatićeva 12, Zagreb
Miroslava Katičić ; University Hospital Merkur, Zajčeva 19, Zagreb; School of Medicine, University of Zagreb, Zagreb, Croatia
Branka Bedenić ; Department of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, Kišpatićeva 12, Zagreb; School of Medicine, University of Zagreb, Zagreb, Croatia
Aleksandra Presečki Stanko ; Department of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, Kišpatićeva 12, Zagreb
Vanda Plečko ; Department of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, Kišpatićeva 12, Zagreb; School of Medicine, University of Zagreb, Zagreb, Croatia

Sažetak

Background: Some of the genes belonging to cag pathogenicity island (cagPAI) in Helicobacter pylori were found to be associated with an increased severity of gastric mucosal inflammation that might lead to the development of gastroduodenal disease.
Aim: The aim of our study was to define a group of patients based on the frequency of virulence genes of cagPAI island and comparison with pathohistological alterations of gastric mucosa who need to be subjected to further eradication therapy after previous unsuccessful eradication therapy and in spite of benign endoscopic findings.
Material and methods: In total 103 H. pylori isolates were analysed. Genes encoding virulence factors were detected by PCR with primers for 10 loci in cagPAI: Apcag (cagA promotor region), cagA1, cagA2, cagA3, cagM, cagT, cagE, LEC, tnpA and tnpB. The patients who provided isolates were classified into three clinical categories: non-ulcer dyspepsia (n=69), erosio/ulcus ventriculi (n=22) and erosio/ulcus duodeni (n=12).
Results: 16 strains (15.5%) were negative for all tested genes. 87 (84.5%) of the isolates had parcially deleated cagPAI. None of the isolates possessed all 10 genes. The frequency of single cagPAI genes were as follows: Apcag 63.1%, cagA1 71.8%, cagA2 69.9%, cagA3 5.8%, cagM 71.8%, cagE 75,7%, cagT 68%, tnpA 9.7%, tnpB 7.8% i LEC 48.5%.
No statistically significant difference was observed between the presence of any cagPAI genes and endosopic diagnosis (p>0.16). The presence of CagA2, Apcag and cagM showed statistically significant correlation with higher level of patohistological parameters of gastritis (p<0.05).
Conclusions: H. pylori isolates with positive cagA, Apcag and cagM genes are correlated to higher degree of patohistological lesions of gastric mucosa; without statistically significant correlation with endoscopic diagnosis.

Ključne riječi

Helicobacter pylori; genotyping; cag pathogenicity island; cagA

Hrčak ID:

156804

URI

https://hrcak.srce.hr/156804

Datum izdavanja:

31.3.2016.

Posjeta: 1.740 *