Croatica Chemica Acta, Vol. 81 No. 1, 2008.
Izvorni znanstveni članak
Effects of Hyperglycemia and Metformin on Expression of Adhesion Molecules on Human Aortic Endothelial Cells
Tatjana Bačun
orcid.org/0000-0001-7012-5325
; Klinička bolnica Osijek, Osijek, Hrvatska
Tatjana Belovari
; Medicinski fakultet, Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska
Mirjana Suver
orcid.org/0000-0002-5418-3178
; Klinička bolnica Osijek, Osijek, Hrvatska
Blaženka Dobrošević
; Klinička bolnica Osijek, Osijek, Hrvatska
Ljubica Glavaš-Obrovac
orcid.org/0000-0001-7497-296X
; Klinička bolnica Osijek, Osijek, Hrvatska; Medicinski fakultet, Sveučilište Josipa Jurja Strossmayera u Osijeku, Osijek, Hrvatska
Sažetak
Expression of adhesion molecules on the endothelial cell surface as a response to elevated glucose concentration is considered as a main event in the development of atherosclerosis. The influence of high glucose concentration and metformin, a wide used anti-diabetic drug, on the expression of E-selectin, vascular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) on human aortic endothelial cells (HAECs) was investigated. HAECs were cultured 4 h in a medium with 5.5, 8.0, 12.0, and 16.5 mmol dm-3 glucose with or without metformin addition. The expression of cell adhesion molecules (CAM) was measured by flow-cytometry. Compared to CAM expression in the medium with 5.5 mmol dm-3 glucose, glucose concentration of 12.0 mmol dm-3 increased expression of E-selectin (62 %), VCAM-1 (four fold) and ICAM-1 (81 %). Metformin administration in the medium with 12.0 mmol dm-3 glucose additionally enhanced E-selectin and VCAM-1 expression compared to effects of corresponding glucose concentration. ICAM-1 expression was only significantly increased in the medium with metformin and 8.0 mmol dm-3 glucose. In conclusion, metformin in condition with elevated glucose concentration additionally increased expression of CAM on HAECs which could contribute to development of macrovascular complications in diabetic patients.
Ključne riječi
cell adhesion molecules; endothelial cells; atherosclerosis; diabetes mellitus; metformin
Hrčak ID:
23442
URI
Datum izdavanja:
15.4.2008.
Posjeta: 1.968 *