Veterinarski arhiv, Vol. 92 No. 2, 2022.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.1368
The protective effect of docosahexaenoic acid on the stomach in Parkinson’s disease induced by MPTP in male C57BL/6 mice
İlknur Birsen
; Department of Chemistry, Faculty of Science, Akdeniz University, Antalya, Turkey
Sema Avcı
; Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
V. Nimet İzgüt-Uysal
; Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
Hakan Soylu
; Department of Histology and Embryology, Faculty of Medicine, Düzce University, Düzce, Turkey
Ayşe Özkan
; Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
Hande Parlak
; Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
Nuray Acar
; Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
İsmail Üstünel
; Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
Gamze Tanrıöver
; Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
Aysel Ağar
; Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey
Sažetak
The purpose of this study was to detect gastric changes in Parkinson’s disease induced by 1-methyl-4-phenyl-1.2.3.6.-tetrahydropyridine (MPTP), and to investigate the protective effect of docosahexaenoic acid (DHA) against these changes in mice. The mice were divided into 4 groups (n=10 in per group) as control, DHA, Parkinson and DHA+Parkinson groups. DHA was administered by gavage for 30 days. On the 23rd day of gavage treatment, the animals of the Parkinson and DHA+Parkinson groups were intraperitoneally injected with MPTP. Seven days after the injection of MPTP, their locomotor activity, bradykinesia and rotarod performance were measured. Tyrosine hydroxylase expression in substantia nigra and the apoptotic index, the concentrations of tumor necrosis factor-α and histamine, and the number of mast cells in the stomach were evaluated. Administration of DHA significantly prevented the reduction in motor functions (P<0.001) and nigral TH neurons (P<0.05), and apoptosis (P<0.05), and an increase in TNF-α concentration (P<0.01) in the stomach. An increase in the number of mast cells in the stomach wall was observed in PD (P<0.001). DHA prevented the increase in the number of mast cells (P<0.05) and the histamine level (P<0.01) due to PD. As a result, MPTP administration in mice caused changes in the stomach as well as impairment in motor functions, and DHA was observed to reduce these changes.
Ključne riječi
docosahexaenoic acid; gastric tissue; MPTP; apoptosis; mast cells; mice
Hrčak ID:
278037
URI
Datum izdavanja:
19.5.2022.
Posjeta: 987 *