Izvorni znanstveni članak

Association of Methylenetetrahydrofolate Reductase (MTHFR-677 and MTHFR-1298) Genetic Polymorphisms with Occlusive Artery Disease and Deep Venous Thrombosis in Macedonians

Igor Spiroski ; Institut za imunobiologiju, Medicinskog fakulteta u Skoplju, Skoplje, Makedonija
Sashko Kedev ; Institut za imunobiologiju, Medicinskog fakulteta u Skoplju, Skoplje, Makedonija
Slobodan Antov ; Institut za imunobiologiju, Medicinskog fakulteta u Skoplju, Skoplje, Makedonija
Todor Arsov ; Institut za imunobiologiju, Medicinskog fakulteta u Skoplju, Skoplje, Makedonija
Marija Krstevska ; Institut za medicinska biokemijska istraživanja, Medicinski fakultet Sveučilišta u Skoplju, Skoplje, Makedonija
Sloboda Dzhekova-Stojkova ; Institut za medicinska biokemijska istraživanja, Medicinski fakultet Sveučilišta u Skoplju, Skoplje, Makedonija
Stojanka Kostovska ; Zavod za transfuziju, Spkoplje, Makedonija
Dejan Trajkov ; Klinika za bolesti srca, Medicinski fakultet Sveučilišta u Skoplju, Makedonija
Aleksandar Petlichkovski ; Institut za imunobiologiju, Medicinskog fakulteta u Skoplju, Skoplje, Makedonija
Ana Strezova ; Institut za imunobiologiju, Medicinskog fakulteta u Skoplju, Skoplje, Makedonija
Olivija Efinska-Mladenovska ; Institut za imunobiologiju, Medicinskog fakulteta u Skoplju, Skoplje, Makedonija
Mirko Spiroski ; Institut za imunobiologiju, Medicinskog fakulteta u Skoplju, Skoplje, Makedonija

Sažetak

Aim To analyze the association of methylenetetrahydrofolate reductase
polymorphisms (MTHFR-677 and MTHFR-1298) with occlusive
artery disease and deep venous thrombosis in Macedonians.
Methods We examined 83 healthy respondents, 76 patients with occlusive
artery disease, and 67 patients with deep venous thrombosis.
Blood samples were collected and DNA was isolated from peripheral
blood leukocytes. Identification of MTHFR mutations was done with
CVD StripAssay (ViennaLab, Labordiagnostika GmbH, Vienna, Austria)
and the population genetics analysis package, PyPop, was used for
the analysis. Pearson P values, crude odds ratio, and Wald’s 95% confidence
intervals were calculated.
Results The frequency of C alleles of MTHFR-677 was 0.575 in patients
with deep venous thrombosis, 0.612 in patients with occlusive
artery disease, and 0.645 in healthy participants. The frequency of T allele
of MTHFR-677 was lower in healthy participants (0.355) than in
patients with occlusive artery disease (0.388) and deep venous thrombosis
(0.425). The frequency of A allele for MTHFR-1298 was 0.729
in healthy participants, 0.770 in patients with occlusive artery disease,
and 0.746 in patients with deep venous thrombosis. The frequency of
C allele of MTHFR-1298 was 0.271 in healthy participants, 0.230 in
patients with occlusive artery disease, and 0.425 in patients with deep
venous thrombosis. No association of MTHFR-677 and MTHFR-
1289 polymorphisms with occlusive artery disease and deep venous
thrombosis was found, except for the protective effect of MTHFR/CA:
CC diplotype for occlusive artery disease.
Conclusion We could not confirm a significant association of MTHFR-
677 and MTHFR-1289 polymorphisms with occlusive artery disease
or deep venous thrombosis in Macedonians, except for the protective
effect of MTHFR/CA:CC diplotype against occlusive artery
disease.

Ključne riječi

MTHFR -677; MTHFR -1298; occlusive artery disease; deep venous thrombosis; Macedonian population

Hrčak ID:

26089

URI

https://hrcak.srce.hr/26089

Datum izdavanja:

15.2.2008.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.648 *