Izvorni znanstveni članak
Inhibition of Lysophosphatidic Acid Receptor-2 Expression by RNA Interference Decreases Lysophosphatidic Acid-induced Urokinase Plasminogen Activator Activation, Cell Invasion, and Migration in Ovarian Cancer SKOV-3 Cells
Gui-li Wang
; Klinika za ginekologiju i porodiljstvo, Shandong pokrajinska bolnica, Kina
Ze-qing Wen
; Klinika za ginekologiju i porodiljstvo, Shandong pokrajinska bolnica, Kina
Wan-peng Xu
; Klinika za opću kirurgiju, bolnički centar Waifang, Kina
Zhi-yu Wang
; Ministarstvo zdravlja, Jinan, Kina
Xue-lian Du
; Klinika za ginekologiju i porodiljstvo, Shandong pokrajinska bolnica, Kina
Fei Wang
; Klinika za ginekologiju i porodiljstvo, Shandong pokrajinska bolnica, Kina
Sažetak
Aim To explore the role of lysophosphatidic acid receptor-2
(LPA2) in regulating lysophosphatidic acid (LPA)-induced urokinase
plasminogen activator (uPA) activation, cell invasion, and
migration in human ovarian cancer cell line SKOV-3.
Methods SKOV-3 cells were stimulated with LPA. Cell supernatant
uPA level and activity were measured using enzyme-linked
immunosorbent assay. LPA2 mRNA expression was inhibited
with LPA2-specific small interfering RNA (siRNA) and examined
using semiquantitative reverse transcriptase-polymerase chain reaction.
LPA-induced cell invasion and migration in transfected
cells were evaluated by a Matrigel invasion chamber and a Transwell
chemotaxis chamber, respectively.
Results LPA stimulation significantly enhanced in vitro uPA activity
in time- and dose-dependent manner. The levels of LPA-induced
uPA protein decreased by 55% in LPA2 siRNA-transfected
cells compared with negatively transfected cells at 24 hours after
being treated with 80 μmol/L LPA (0.75 ± 0.03 vs 0.34 ± 0.04,
P = 0.004). In the LPA2 specific siRNA-transfected SKOV-3 cells,
LPA treatment at 80 μmol/L induced considerably less invasion
and migration compared with negative control siRNA-transfected
SKOV-3 cells (invasion: 178 ± 17.2 vs 36.2 ± 3.3, P = 0.009;
migration: 220.4 ± 25.5 vs 57 ± 7.6, P = 0.009).
Conclusion LPA2 has an essential role in LPA-induced uPA activation
and tumor cell invasion in ovarian cancer SKOV-3 cells.
Ključne riječi
RNA; Small Interfering/CD44/Ovarian Neoplasms/in vitro/Animal Experimentation
Hrčak ID:
26101
URI
Datum izdavanja:
15.4.2008.
Posjeta: 1.951 *