Šumarski list, Vol. 149 No. 1-2, 2025.
Izvorni znanstveni članak
https://doi.org/10.31298/sl.149.1-2.2
Variations in the total phenolic content and antioxidant activity of fresh houseleek leaves (Sempervivum tectorum L.) with regard to provenances from urban and rural environments - the area of Mostar, Bosnia and Herzegovina
Esmera Kajtaz
; Farmaceutski fakultet Univerziteta u Sarajevu, Bosna i Hercegovina
Dženita Alibegić
; Farmaceutski fakultet Univerziteta u Sarajevu, Bosna i Hercegovina
Haris Nikšić
; Farmaceutski fakultet Univerziteta u Sarajevu, Bosna i Hercegovina
Željko Španjol
; Sveučilište u Zagrebu, Fakultet šumarstva i drvne tehnologije, Zagreb, Hrvatska
Boris Dorbić
; Sveučilište u Splitu, Samostalni studij Mediteranska poljoprivreda, Split
Sažetak
This research aimed to determine the antioxidant activity and total phenolic
content of fresh leaves of Sempervivum tectorum concerning provenances from
urban and rural environments. Fresh leaves of the houseleek plant material
were collected on a sunny day in June 2024. The homogenized sample was then
subjected to maceration extraction at room temperature using distilled water
and aqueous solutions of 70% and 50% ethanol and 70% and 50% methanol.
The total phenolic content was determined by the Folin-Ciocalteu method,
and the antioxidant activity was evaluated by the DPPH scavenging method
and expressed using the Trolox standard as an equivalent (μmol TE/g). Total
phenolic content ranged from 42.91 ± 0.83 to 115.96 ± 0.48 mg GAE/g, depending
on the type of solvent used and the origin of the plant material.
The highest values of antioxidant activity had the methanol extract with a value
of 672.51 ± 2.47 μmol TE/g, and the lowest values had aqueous extracts of plant
material of both origins. The houseleek samples from higher altitudes showed
exceptional antioxidant activity with values from 672.51 ± 2.47 to 359.79 ± 3.97
μmol TE/g, while the samples collected at lower altitudes showed a lower
ability to remove radicals according to the DPPH method with values from
366.86 ± 6.91 to 232.78 ± 4.55 μmol TE/g. Using multiple linear regression, it was
shown that provenance (β = -0.64; t = -3.167; 0.0016 < 0.05) and solvent (β = 0.552;
t = 2.734; 0.029 < 0.05) are significant predictors of the total phenolic content for
the analyzed samples. Provenance (β = 0.78; t = 4.55; 0.003 < 0.05) and solvent (β
= -0.422; t = 2.445; 0.044 < 0.05) are also significant predictors for the antioxidant
activity results of the analyzed samples. The result of the t-test showed that
there is a statistically significant difference (0.013 < 0.050) in antioxidant activity
regarding the provenance of the plant material (urban/rural).
Ključne riječi
Sempervivum tectorum, Folin-Ciocalteu method, total phenols, DPPH method, antioxidant activity
Hrčak ID:
326138
URI
Datum izdavanja:
28.2.2025.
Posjeta: 0 *