Original scientific paper

https://doi.org/10.24099/vet.arhiv.2540

Evaluation of the antibacterial activities of Ag/ZnO nanoparticles against Streptococcus agalactiae, Staphylococcus aureus and Escherichia coli isolated from infected bovine mammary glands

Shahrdad Arastoo ; Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
Zeinab Sharafi ; Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Pegah Shakib ; Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Babak Mohammadian ; Professor of Department of Pathology Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
Mohammad Reza Zolfaghari ; Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran *

* Corresponding author.

Abstract

The objective of this study was to evaluate the antibacterial activity of Ag/ZnO nanoparticles against Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae isolated from bovine mastitis in dairy cows, both in vivo and in vitro. Ag/ZnO nanoparticles were synthesized by means of the thermal decomposition of oxalate precursors. SEM images revealed that the nanoparticles had a size range of 10-50 nm. A toxicity assessment of nanoparticles conducted using the Artemia salina model indicated that the nanoparticles were not toxic at high concentrations (LD50>6384 μg/ml). A total of 138 mastitic milk samples were cultured to assess the antimicrobial effects of Ag/ZnO NPs. The antimicrobial susceptibility of the isolated bacteria was assessed by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-kill test. To evaluate the efficacy of the treatment, the somatic cell count (SCC), California mastitis test (CMT), and histological tests were employed to assess the cow udders with mastitis that had been treated with Ag/ZnO NPs. The most frequently isolated pathogen was S. agalactiae (50.72%), which demonstrated the highest susceptibility to Ag/ZnO NPs in vitro and in vivo tests.

Keywords

mastitis; antimicrobial effect; dairy cow; silver-zinc oxide nanoparticles (Ag/ZnO NPs)

Hrčak ID:

330422

URI

https://hrcak.srce.hr/330422

Publication date:

1.4.2025.

Article data in other languages: croatian

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