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Detection of enrofloxacin residues in chicken meat by microbiological (growth inhibition test) and ELISA method after experimental prophylactic and therapeutic application

Enida Članjak ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Muhamed Smajlović ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Faruk Čaklovica ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Davor Alagić ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Kenan Čaklovica ; Department of Food Hygiene and Technology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina
Ahmed Smajlović ; Department of Pharmacology and Toxicology, Veterinary faculty University of Sarajevo, Zmaja od Bosne 90, 71 000 Sarajevo, Bosnia and Herzegovina

Puni tekst: engleski, pdf (204 KB) str. 198-205 preuzimanja: 1.203* citiraj
APA 6th Edition
Članjak, E., Smajlović, M., Čaklovica, F., Alagić, D., Čaklovica, K. i Smajlović, A. (2011). Detection of enrofloxacin residues in chicken meat by microbiological (growth inhibition test) and ELISA method after experimental prophylactic and therapeutic application. MESO: Prvi hrvatski časopis o mesu, XIII (3), 198-205. Preuzeto s https://hrcak.srce.hr/72580
MLA 8th Edition
Članjak, Enida, et al. "Detection of enrofloxacin residues in chicken meat by microbiological (growth inhibition test) and ELISA method after experimental prophylactic and therapeutic application." MESO: Prvi hrvatski časopis o mesu, vol. XIII, br. 3, 2011, str. 198-205. https://hrcak.srce.hr/72580. Citirano 12.07.2020.
Chicago 17th Edition
Članjak, Enida, Muhamed Smajlović, Faruk Čaklovica, Davor Alagić, Kenan Čaklovica i Ahmed Smajlović. "Detection of enrofloxacin residues in chicken meat by microbiological (growth inhibition test) and ELISA method after experimental prophylactic and therapeutic application." MESO: Prvi hrvatski časopis o mesu XIII, br. 3 (2011): 198-205. https://hrcak.srce.hr/72580
Harvard
Članjak, E., et al. (2011). 'Detection of enrofloxacin residues in chicken meat by microbiological (growth inhibition test) and ELISA method after experimental prophylactic and therapeutic application', MESO: Prvi hrvatski časopis o mesu, XIII(3), str. 198-205. Preuzeto s: https://hrcak.srce.hr/72580 (Datum pristupa: 12.07.2020.)
Vancouver
Članjak E, Smajlović M, Čaklovica F, Alagić D, Čaklovica K, Smajlović A. Detection of enrofloxacin residues in chicken meat by microbiological (growth inhibition test) and ELISA method after experimental prophylactic and therapeutic application. MESO: Prvi hrvatski časopis o mesu [Internet]. 2011 [pristupljeno 12.07.2020.];XIII(3):198-205. Dostupno na: https://hrcak.srce.hr/72580
IEEE
E. Članjak, M. Smajlović, F. Čaklovica, D. Alagić, K. Čaklovica i A. Smajlović, "Detection of enrofloxacin residues in chicken meat by microbiological (growth inhibition test) and ELISA method after experimental prophylactic and therapeutic application", MESO: Prvi hrvatski časopis o mesu, vol.XIII, br. 3, str. 198-205, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/72580. [Citirano: 12.07.2020.]
Puni tekst: hrvatski, pdf (210 KB) str. 167-174 preuzimanja: 333* citiraj
APA 6th Edition
Članjak, E., Smajlović, M., Čaklovica, F., Alagić, D., Čaklovica, K. i Smajlović, A. (2011). Detekcija ostataka enrofloksacina u kozumnom pilećem mesu mikrobiološkom (test inhibicije rasta) i ELISA metodom nakon eksperimentalne profilaktičke i terapijske aplikacije. MESO: Prvi hrvatski časopis o mesu, XIII (3), 167-174. Preuzeto s https://hrcak.srce.hr/72580
MLA 8th Edition
Članjak, Enida, et al. "Detekcija ostataka enrofloksacina u kozumnom pilećem mesu mikrobiološkom (test inhibicije rasta) i ELISA metodom nakon eksperimentalne profilaktičke i terapijske aplikacije." MESO: Prvi hrvatski časopis o mesu, vol. XIII, br. 3, 2011, str. 167-174. https://hrcak.srce.hr/72580. Citirano 12.07.2020.
Chicago 17th Edition
Članjak, Enida, Muhamed Smajlović, Faruk Čaklovica, Davor Alagić, Kenan Čaklovica i Ahmed Smajlović. "Detekcija ostataka enrofloksacina u kozumnom pilećem mesu mikrobiološkom (test inhibicije rasta) i ELISA metodom nakon eksperimentalne profilaktičke i terapijske aplikacije." MESO: Prvi hrvatski časopis o mesu XIII, br. 3 (2011): 167-174. https://hrcak.srce.hr/72580
Harvard
Članjak, E., et al. (2011). 'Detekcija ostataka enrofloksacina u kozumnom pilećem mesu mikrobiološkom (test inhibicije rasta) i ELISA metodom nakon eksperimentalne profilaktičke i terapijske aplikacije', MESO: Prvi hrvatski časopis o mesu, XIII(3), str. 167-174. Preuzeto s: https://hrcak.srce.hr/72580 (Datum pristupa: 12.07.2020.)
Vancouver
Članjak E, Smajlović M, Čaklovica F, Alagić D, Čaklovica K, Smajlović A. Detekcija ostataka enrofloksacina u kozumnom pilećem mesu mikrobiološkom (test inhibicije rasta) i ELISA metodom nakon eksperimentalne profilaktičke i terapijske aplikacije. MESO: Prvi hrvatski časopis o mesu [Internet]. 2011 [pristupljeno 12.07.2020.];XIII(3):167-174. Dostupno na: https://hrcak.srce.hr/72580
IEEE
E. Članjak, M. Smajlović, F. Čaklovica, D. Alagić, K. Čaklovica i A. Smajlović, "Detekcija ostataka enrofloksacina u kozumnom pilećem mesu mikrobiološkom (test inhibicije rasta) i ELISA metodom nakon eksperimentalne profilaktičke i terapijske aplikacije", MESO: Prvi hrvatski časopis o mesu, vol.XIII, br. 3, str. 167-174, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/72580. [Citirano: 12.07.2020.]

Sažetak
The paper presents detection results of enrofloxacin residues in muscle tissue and liver tissue of broiler chickens after the experimental application of prophylactic and therapeutic doses of the investigated drug. Two methods were used for the detection of enrofloxacin residues in muscle and liver tissue, and they are: microbiological method (growth inhibition test) and ELISA test. The aim of this research was to examine the reliability of the microbiological growth inhibition test (diffusion method) with the application of reference strain E. coli ATCC 10 536 as the microorganism- test for the detection of enrofloxacin residues in broiler meat and to compare the applied methods. By using phi correlation coefficient it was determined that there is a statistically very significant positive correlation (p <<0.001) between the data of the microbiological and ELISA method and in samples of muscle tissue and liver tissue. There was also determined that, in experimental conditions, both microbiological and ELISA method achieve equally positive results in the detection
of the allowed quantities of enrofloxacin residues, although they are different measures (mm or ppb) of the same phenomenon.

Ključne riječi
enrofloxacin; growth inhibition test; ELISA

Hrčak ID: 72580

URI
https://hrcak.srce.hr/72580

[hrvatski]

Posjeta: 1.746 *