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In-Vitro Studies on the Antioxidant Assay Profiling of Root of Withania somnifera L. (Ashwagandha) Dunal: Part 2

Ajay Pal ; Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore – 570 011, India
Mahadeva Naika ; Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore – 570 011, India
Farhath Khanum ; Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore – 570 011, India
Amarinder Singh Bawa ; Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore – 570 011, India

Puni tekst: engleski, pdf (179 KB) str. 95-101 preuzimanja: 306* citiraj
APA 6th Edition
Pal, A., Naika, M., Khanum, F. i Bawa, A.S. (2012). In-Vitro Studies on the Antioxidant Assay Profiling of Root of Withania somnifera L. (Ashwagandha) Dunal: Part 2. Agriculturae Conspectus Scientificus, 77 (2), 95-101. Preuzeto s https://hrcak.srce.hr/83137
MLA 8th Edition
Pal, Ajay, et al. "In-Vitro Studies on the Antioxidant Assay Profiling of Root of Withania somnifera L. (Ashwagandha) Dunal: Part 2." Agriculturae Conspectus Scientificus, vol. 77, br. 2, 2012, str. 95-101. https://hrcak.srce.hr/83137. Citirano 28.10.2021.
Chicago 17th Edition
Pal, Ajay, Mahadeva Naika, Farhath Khanum i Amarinder Singh Bawa. "In-Vitro Studies on the Antioxidant Assay Profiling of Root of Withania somnifera L. (Ashwagandha) Dunal: Part 2." Agriculturae Conspectus Scientificus 77, br. 2 (2012): 95-101. https://hrcak.srce.hr/83137
Harvard
Pal, A., et al. (2012). 'In-Vitro Studies on the Antioxidant Assay Profiling of Root of Withania somnifera L. (Ashwagandha) Dunal: Part 2', Agriculturae Conspectus Scientificus, 77(2), str. 95-101. Preuzeto s: https://hrcak.srce.hr/83137 (Datum pristupa: 28.10.2021.)
Vancouver
Pal A, Naika M, Khanum F, Bawa AS. In-Vitro Studies on the Antioxidant Assay Profiling of Root of Withania somnifera L. (Ashwagandha) Dunal: Part 2. Agriculturae Conspectus Scientificus [Internet]. 2012 [pristupljeno 28.10.2021.];77(2):95-101. Dostupno na: https://hrcak.srce.hr/83137
IEEE
A. Pal, M. Naika, F. Khanum i A.S. Bawa, "In-Vitro Studies on the Antioxidant Assay Profiling of Root of Withania somnifera L. (Ashwagandha) Dunal: Part 2", Agriculturae Conspectus Scientificus, vol.77, br. 2, str. 95-101, 2012. [Online]. Dostupno na: https://hrcak.srce.hr/83137. [Citirano: 28.10.2021.]

Sažetak
The anti-oxidative activities of six different extracts of Withania somnifera (Ashwagandha) root, prepared in a sequential manner starting from non-polar (hexane) to polar (water) solvent, were investigated employing various established in-vitro systems that include total antioxidant activity (TAA), total reducing power (TRP), nitric oxide scavenging activity (NOSA) and lipid peroxidation inhibition activity (LPIA). Among all the extracts, methanol extract was found the most potent and additionally, its DNA damage protective efficacy was tested using pRSET-A vector system. Positive correlations were established between total polyphenolic contents (TPC) and various activities strongly suggesting that the observed activities of the extracts may be ascribed to their phenolic compounds that could be responsible, at least partly, for the observed antioxidant activities. Six main compounds viz. alkaloids, hydroxybenzene, terpene ansteroid, saponin, organic acids and flavone were identified in methanol extract using thin layer chromatography (TLC) while by employing reverse-phase high pressure liquid chromatography (RP-HPLC) four polyphenols namely epicatechin (3.21 μg/g), quercetin-3-rhamnoside (1.12 μg/g), gallic acid (0.05 μg/g) and rutin hydrate (0.01 μg/g) were identified and quantified in aforementioned extract. Overall, the results of study clearly demonstrated that methanolic extract of Ashwagandha root possesses a marked antioxidant activity.

Ključne riječi
reducing potential; free radicals; DNA damage; polyphenols

Hrčak ID: 83137

URI
https://hrcak.srce.hr/83137

Posjeta: 682 *